Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation
Figure 1
Higher proportion of MSCs can be isolated from the membrane compare to the placenta. Fetal tissue derived MSCs display greater proliferation kinetic than bone marrow derived MSCs. (a) MSCs were defined as CD45−, CD34−, CD29+, CD90+, CD73+, and CD105+. Their proportion was then calculated in freshly digested placenta specimens and fetal membranes form 3 different donors. MSCs represented 15.67% (±0.29%) and 2.14% (±0.65%) of cells isolated from the membrane and placenta, respectively (**). (b) Day 1 phase contrast microscopy of adherent cells from placenta and membrane directly plated after tissue digestion in MSCs media. We can see significantly more adherent cells from digested membrane compared to placenta. (c) MSCs derived from placenta and membranes were expanded up to passage 15 without changes of their morphology or proliferation rate. Proliferation rate was assessed by cell counting at different passages. It was similar in placenta- and membrane-derived MSCs with no differences between early and late passages. However, proliferation rate was significantly higher than proliferation rate of bone-marrow-derived MSCs.
