Development of a Functional Schwann Cell Phenotype from Autologous Porcine Bone Marrow Mononuclear Cells for Nerve Repair

<table>BM-MNC-derived Schwann cells increase <svg height="20.0625" id="M56" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 47.962502 20.0625" width="47.962502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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<g transform="matrix(.012,-0,0,-.012,24.85,7.613)"><use xlink:href="#x32"></use></g><g transform="matrix(.012,-0,0,-.012,30.561,7.613)"><use xlink:href="#x2B"></use></g>
<g transform="matrix(.017,-0,0,-.017,38.162,15.775)"><use xlink:href="#x5D"></use></g>
<g transform="matrix(.012,-0,0,-.012,44.025,19.863)"><use xlink:href="#x1D456"></use></g>
</svg> in response to UTP or ATP.  BM-MNC-derived Schwann cells were loaded with Fluo-4 then either 25 <svg height="12.175" id="M57" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 9.5625 12.175" width="9.5625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><use xlink:href="#x1D707"></use></g>
</svg>M UTP or ATP was added (large arrow).  Note the near identical nucleotide agonist-induced <svg height="20.0625" id="M58" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 47.962502 20.0625" width="47.962502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,15.775)"><use xlink:href="#x5B"></use></g><g transform="matrix(.017,-0,0,-.017,5.927,15.775)"><use xlink:href="#x43"></use></g><g transform="matrix(.017,-0,0,-.017,17.367,15.775)"><use xlink:href="#x61"></use></g>
<g transform="matrix(.012,-0,0,-.012,24.85,7.613)"><use xlink:href="#x32"></use></g><g transform="matrix(.012,-0,0,-.012,30.561,7.613)"><use xlink:href="#x2B"></use></g>
<g transform="matrix(.017,-0,0,-.017,38.162,15.775)"><use xlink:href="#x5D"></use></g>
<g transform="matrix(.012,-0,0,-.012,44.025,19.863)"><use xlink:href="#x1D456"></use></g>
</svg> response to both nucleotides suggesting the presence of a P2Y purinergic receptor.</table>

Stem Cells International

fig7

Figure 7

Figure 7: Development of a Functional Schwann Cell Phenotype from Autologous Porcine Bone Marrow Mononuclear Cells for Nerve Repair 