Development of a Functional Schwann Cell Phenotype from Autologous Porcine Bone Marrow Mononuclear Cells for Nerve Repair

<table>Photographs taken from confocal live-cell imaging of Fluo-4 loaded BM-MNC-derived Schwann cells before (a) and after (b) the addition of 25 <svg height="12.175" id="M59" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 9.5625 12.175" width="9.5625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><use xlink:href="#x1D707"></use></g>
</svg>M ATP for 3 minutes.  Note the rapid ATP-induced increase in cytoplasmic <svg height="20.0625" id="M60" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 47.962502 20.0625" width="47.962502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,15.775)"><use xlink:href="#x5B"></use></g><g transform="matrix(.017,-0,0,-.017,5.927,15.775)"><use xlink:href="#x43"></use></g><g transform="matrix(.017,-0,0,-.017,17.367,15.775)"><use xlink:href="#x61"></use></g>
<g transform="matrix(.012,-0,0,-.012,24.85,7.613)"><use xlink:href="#x32"></use></g><g transform="matrix(.012,-0,0,-.012,30.561,7.613)"><use xlink:href="#x2B"></use></g>
<g transform="matrix(.017,-0,0,-.017,38.162,15.775)"><use xlink:href="#x5D"></use></g>
<g transform="matrix(.012,-0,0,-.012,44.025,19.863)"><use xlink:href="#x1D456"></use></g>
</svg> as indicated by the increase in cellular fluorescence (red) (b);  (c, d) represent intensity maps of the relative increase in <svg height="20.0625" id="M61" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 47.962502 20.0625" width="47.962502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,15.775)"><use xlink:href="#x5B"></use></g><g transform="matrix(.017,-0,0,-.017,5.927,15.775)"><use xlink:href="#x43"></use></g><g transform="matrix(.017,-0,0,-.017,17.367,15.775)"><use xlink:href="#x61"></use></g>
<g transform="matrix(.012,-0,0,-.012,24.85,7.613)"><use xlink:href="#x32"></use></g><g transform="matrix(.012,-0,0,-.012,30.561,7.613)"><use xlink:href="#x2B"></use></g>
<g transform="matrix(.017,-0,0,-.017,38.162,15.775)"><use xlink:href="#x5D"></use></g>
<g transform="matrix(.012,-0,0,-.012,44.025,19.863)"><use xlink:href="#x1D456"></use></g>
</svg> of the BM-MNC-derived Schwann cell by 25 <i>μ</i>M ATP as shown in (a, b), respectively.</table>

Stem Cells International

fig8

Figure 8

Figure 8: Development of a Functional Schwann Cell Phenotype from Autologous Porcine Bone Marrow Mononuclear Cells for Nerve Repair 