Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency
Figure 2
Histological evidence of germ layer differentiation in embryoid bodies generated from human pluripotent stem cells cultured by different methods to illustrate equivalence of culture techniques [63]. Shown are images of hematoxylin and eosin-stained histologic sections of EBs from hESCs propagated directly on mouse embryonic fibroblast (MEF) feeder layer (top row, a–c) or hESCs propagated in indirect coculture with MEFs (second row, d–f), hiPSCs propagated on MEFs (third row, g–i), or hiPSCs propagated in indirect coculture with MEFs (bottom row, j–l). Equivalent trilineage potential is demonstrated by presence of ectodermal (neuroepithelial) (a, d, g, and j); mesodermal (fibrous connective) (b, e, h, and k), and endodermal (intestinal) (c, f, i, and l) differentiation in these EBs. Arrows point to the corresponding tissue in each figure. Magnification is 400x total (10x ocular, 40x objective). Each scale bar represents 50 μm in length.
