Review Article
Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency
Table 1
Common techniques to form human embryoid bodies.
| | Controls EB size | Need for single cell suspension | Large-scale bioreactor production | Need for special equipment | References |
| Heterogeneous methods | | | | | | Liquid suspension culture | No | No | No | No | [15, 16] | Stirred flask culture | No | No | Yes | Yes | [27] | Rotary cell culture systems (RCCSS) | No | No | Yes | Yes | [28, 29] |
| Homogeneous methods | | | | | | Hanging drop culture | Yes | Yes | No | No | [13, 30, 31] | Low adhesion U-bottom multiwell plates | Yes | No | No | No | [14] | Indented solid microsurfaces (AggrewellTM) | Yes | Yes | No | Yes | [32] |
| Other methods | | | | | | Hydrogels (e.g., methylcellulose, agarose, alginate) | No | Yes | No | No | [15, 33, 34] |
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