Review Article
Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency
Table 2
Typical markers for the analysis of human embryoid bodies.
| | Marker name | Alternate name | Marker type | Suitable marker for gene expression | Suitable marker for IHC | Reference |
| Pluripotency | | | | | | | | Oct4 (POU5F1) | Octamer-binding transcription factor 4 | Transcription factor | Yes | Yes | [44] | | Nanog | n/a | Transcription factor | Yes | Yes | [45] | | REX-1 | Zinc finger protein 42 homolog (ZFP42) | Transcription factor | Yes | Yes | [2] | | SOX-2 | SRY (sex determining region Y)-box 2 | Transcription factor | Yes | Yes | [46] | | SSEA-3 | Stage-specific embryonic antigen 3 (SSEA-3) | Surface antigen | No | Yes | [47] | | SSEA-4 | Stage-specific embryonic antigen 3 (SSEA-4) | Surface antigen | No | Yes | [47] | | Tra-I-60 | n/a | Surface antigen | No | Yes | [48] | | Tra-I-81 | n/a | Surface antigen | No | Yes | [48] |
| Ectoderm | | | | | | | | GFAP | Glial fibrillary acidic protein | Glial intermediate filament | Yes | Yes | [49] | | Nestin | n/a | Intermediate filament | Yes | Yes | [49] | | Pax-6 | Paired box gene 6 | Transcription factor | Yes | Yes | [50] | | Sox-1 | Sex determining region Y-box 1 | Transcription factor | Yes | Yes | [51] |
| Endoderm | | | | | | | | AFP | Alpha-fetoprotein | Plasma Protein | Yes | No | [52] | | Amylase | Alpha-amylase | Metabolic Enzyme | Yes | Yes | [53] | | FOXA2 | Hepatocyte nuclear factor 3-beta (HNF-3B) | Transcription Factor | Yes | Yes | [54] | | PDX1 | Pancreatic and duodenal homeobox 1, insulin promoter factor 1 | Transcription factor | Yes | Yes | [55] | | GATA4 | GATA binding protein 4 | Transcription factor | Yes | Yes | [56] |
| Mesoderm | | | | | | | | Brachyury | n/a | Transcription factor | Yes | Yes | [57] | | CD34 | Cluster of differentiation molecule 34 | Surface antigen | No | Yes | [58] | | FLT1 | Vascular endothelial growth factor receptor 1 | Surface receptor | Yes | Yes | [59] | | RUNX1 | AML1, Runt-related transcription factor 1 | Transcription factor | Yes | Yes | [60] |
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