Adipose-Derived Mesenchymal Stromal/Stem Cells: Tissue Localization, Characterization, and Heterogeneity

Table 1

Summary of cell culture parameters which affect the undifferentiated state of ASCs.

(i) Basal medium	→ Medium composition (e.g., DMEM, αMEM, M199)
	→ Glucose content
	→ Calcium content
(ii) Supplements	→ Serum (bovine or human)
	→ Platelet lysate
	→ Growth factors (e.g., bFGF, aFGF, EGF, PDGF)
	→ Corticoids
	→ Antioxidants
	→ Antibiotics (?)
(iii) Environment	→ Hypoxia
	→ Perfusion culture (Shear stress)
	→ Stiffness of the substrate (coating)
	→ Mechanical strain
	→ Confluency (cell cell-contacts)