Development of a Vascularized Skin Construct Using Adipose-Derived Stem Cells from Debrided Burned Skin

<table>(a) Discarded burn skin. (b–d) Immunohistochemical images of section from discarded skin biopsies: (b) stained with PDGFR<i>β</i> (Alexa fluor 488), (c) Hoechst for nuclei, and (d) overlay of PDGFR<i>β</i> and Hoechst. (e) Stem cell isolation process from the adipose tissue layer of discarded burn skin (dsASCs) using the Transpose RTtissue processing system. (f) Fluorescent-activated cell sorting (FACS) analysis of P1 dsASCs stained with phycoerythrin-labeled PDGFR<i>β</i>. (g–i) Immunocytochemical images of P1 dsASCs: (g) stained with PDGFR<i>β</i> (Alexafluor 594), (h) Hoechst for nuclei, and (i) overlay of PDGFR<i>β</i> and Hoechst. Original magnification: ×200 (b–d and dsASCs in (e)) and ×600 (g–i).</table>

Stem Cells International

fig3

Figure 3

Figure 3: Development of a Vascularized Skin Construct Using Adipose-Derived Stem Cells from Debrided Burned Skin 