Expression of SIRT1 and DBC1 in Developing and Adult Retinas
Figure 4
(a) Light microscope image of hRPCs immunostained for SIRT1. Immunoreactivity can be seen predominantly in the cytoplasm but also in the nuclei of some cells. (b) hRPCs immunostained for DBC1. Immunoreactivity can be seen predominantly in the nuclei but also in the cytoplasm of some cells. (c) Distribution of SIRT1 and DBC1 staining in hRPCs. (d) SIRT1 staining classification and hRPC cell size. Of note, SIRT1 positivity was observed in both the cytoplasm and nucleus in hRPCs of all sizes. No significant difference in SIRT1 distribution was observed for small versus medium/large cells. (e) DBC1 staining classification and hRPC cell size. Of note, DBC1 positivity in the cytoplasm was more frequently seen in small cells compared to medium/large cells. Also, some medium/large cells were negative for DBC1 whereas all small cells were DBC1 positive.
