Multipotent Mesenchymal Stromal Stem Cell Expansion by Plating Whole Bone Marrow at a Low Cellular Density: A More Advantageous Method for Clinical Use

Table 3

Analyses of seeding density effects in terms of cumulative PD.

1st passage
𝑁=4 (𝑃=0.068)	10 cells/cm^2	100 cells/cm^2	500 cells/cm^2	1000 cells/cm^2
Median	5.28	3.27	2.08	2.28
Minim	2.49	.68	.58	1.39
Maxim	5.57	4.65	3.07	2.63
2nd passage
𝑁=4   (𝑃=0.001)^∗	10 cells/cm^2	100 cells/cm^2	500 cells/cm^2	1000 cells/cm^2
Median	10.45	6.82	4.48	4.48
Minim	8.33	4.76	2.90	2.88
Maxim	12.06	10.15	6.99	5.94
3rd passage
𝑁=4 (𝑃=0.017)^∗	10 cells/cm^2	100 cells/cm^2	500 cells/cm^2	1000 cells/cm^2
Median	14.85	9.89	8.14	6.37
Minim	13.41	6.78	3.82	4.92
Maxim	18.78	13.69	9.84	8.52