Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Figure 2
Improved uniformity of stem cell cultures by laser-mediated passage. (a) Brightfield images of iPSC cultures 2 days after laser-mediated passage (200 μm sections, left) or collagenase passage (right). Colonies are shown by red outline. Scale bar, 1 mm. (b) Alkaline phosphatase (AP) staining of iPSC colonies 1 day after laser-mediated passage (200 μm sections, left) or collagenase passage (right). Scale bar, 1 mm. (c) Colony size of iPSC cultures on days 1 and 3 after laser-mediated passage (200 μm sections), StemPro EZPassage Disposable Stem Cell Passaging Tool (EZ Passage), manual passage using a pipette tip, collagenase treatment, or trypsin dissociation of cells. Longest diameter of each colony was manually measured using brightfield images (top, colonies per data point). Number of cells per colony was manually counted using Hoechst stained cultures (bottom, colonies per data point). Data are shown as scatter plot with red line indicating mean. The CV is shown in red text above each sample. Asterisks (*) indicate variances that are statistically significant when compared to laser-mediated passage using ANOVA, with a considered significant.
