Research Article
Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Table 1
Regions in H9 human ESCs with genomic abberations as determined by aCGH (corresponding schematic is shown in Figure 4(e)). No subkaryotypic alterations were detected in hESCs propagated for 24 consecutive passages (H9 P59) relative to the starting hESC population (H9 P35), suggesting that the genome of laser-mediated passaged cells is both normal and stable. Data is reported using genome built HG18. ratios ≥ 0.6 are amplifications (amp) or ≤−1.0 are deletions (del) found in all cells. ratios < 0.6 or >−1.0 represent mosaicism within the culture.
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