Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells

Figure 1

Characterization of MSCs according to ISCT guidelines. Panel 1: spindle-shaped cells typical of MSCs after 24 hrs, 72 hrs, and 5 days from seeding. Panel 2: immunophenotypic analysis of MSCs showing the negativity of CD34, CD45 and CD14 expression and the positivity of CD90, CD73, CD105, and CD29. Panel 3: differentiative potential of a representative MSC: presence of calcium ossalates observed in Van Kossa staining (a) after osteogenic induction; presence of lipid intracytoplasmic vacuoles stained with Oil Red O (b) after adipogenic induction and presence of hjaluronic acid by Alcian Blue staining (c) after chondrogenic induction. Original magnification 40× (a, c) and 20× (b).