Markers for Characterization of Bone Marrow Multipotential Stromal Cells

Table 1

Surface antigen expression on cultured MSCs from different species.

Surface antigen	Human	Mouse**	Rat	Rabbit	Primate	Dog	Pig	Goat	Sheep	Cow	Horse
CD13	++ [72]	− [73]*			NC [72]	NC [72]	NC [72]	NC [72]	NC [72]	NC [74]
	++ [75]	+ [76]*
	++ [77]
CD29	++ [72]	++ [78]*	++ [79]*	++ [80]	NC [72]	NC [72]	NC [72]	NC [72]	NC [72]		++ [59]
	++ [81]	++ [73]*	++ [82]*			+ [83]	++ [81]		++ [84]*		++ [62]*
	++ [6]		++ [58]*				++ [46]		++ [85]*
	++ [75]						++ [47]
	++ [77]
CD31	− [72]	− [78]*			NC [72]	NC [72]	NC [72]	NC [72]	NC [72]
	− [81]	− [86]*					− [81]		− [84]*
	− [75]						− [46]		− [85]*
CD34	− [72]	− [78]*		+− [80]	NC [72]	NC [72]	NC [72]	NC [72]	− [61]		−(CND) [59]
	− [81]	+− [73]*			− [87]	− [83]*	− [81]		NC [72]
	− [6]	− [76]*
	− [9]	+ [86]*
CD44	++ [72]	++ [78]*	+ [82]*		NC [72]	NC [72]	NC [72]	NC [72]	++ [61]	++ [74]	− [59]
	++ [81]	++ [73]*				+ [83]*	++ [81]		NC [72]		+ [62]*
	++ [6]						++ [46]		++ [84]
	++ [77]								++ [85]
CD49e	++ [81]	++ [73]*	++ [79]*				− (CND) [81]
	++ [77]
CD45	− [72]	− [78]*	− [79]*		− [87]	NC [72]	NC [72]	NC [72]	− [61]*	+− [74]*	− (CND) [59]
	− [81]	+− [73]*	+− [82]*			− [83]*	− [81]*		NC [72]
	− [6]	− [76]*	+− [58]*				− [47]		− [84]
	− [9]	− [86]*
CD73	++ [72]	+− [73]*	++[79]*		++ [72]	− [72]	− [72]	− [72]	− [72]	NC [74]	− [59]
	++ [9]		++[88]*
	++ [75]
	++ [77]
CD90	++ [72]	++ [73]*	++[79]*		++ [72]	− [72]	++ [72]	− [72]	− [72]		++ [59]
	++ [81]	+ [76]*	++[82]*			+ [83]	++ [81]
	++ [6]	− [86]*	++ [58]*				++ [46]
	++ [9]		++ [88]*				++ [47]
	++ [77]
CD105	++ [72]	+ [78]*	++ [79]*		++ [72]	− [72]	− [72]	− [72]	+− [61]	NC [74]	− [59]
	++ [81]	++ [73]*			+ [87]	− [83]	NC [81]		− [72]
	++ [6]						++ [47]
	++ [9]
	++ [77]
CD146	++ [72]				++ [72]	+ [72]	++ [72]	− [72]	++ [72]
	++ [81]						+ [81]
	++ [77]
CD166	++ [81]	+− [73]*					− [81]		++ [85]	++ [74]
	++ [75]
	++ [77]
CD271	+− [72]				+ [72]	+− [72]	+− [72]	+− [72]	+− [72]
	+− [81]						+− [81]
c-Kit (CD117)	− [81]	++ [78]*					− [81]
		+− [73]*
		− [76]*
		− [86]*
Sca-1	− [72]	++ [78]*			− [72]	− [72]	− [72]	− [72]	− [72]
	− [81]	++ [73]*					− [81]
		++ [76]*
		++ [86]*
SSEA4	++ [72]	++ [89]*			++ [72]	− [72]	− [72]	+ [72]	− [72]
	++ [81]						NC [81]
	++ [89]
	++ [77]
Stro-1	++ [81]		++[79]*				+− [81]		− [85]
	++ [26]
W8-B2/ MSCA-1	+ [72]				+ [72]	+ [72]	+ [72]	++ [72]	+− [72]
	+ [81]						+ [81]

*Species-specific antibody (all others are antihuman antibodies). NC: no cross-reactivity; CND: cross-reactivity not determined. Symbols indicate marker expression levels: −: no expression; +−: <5% expression; +: 5−50% expression, ++: 50−100% expression. **Markers specific for MSCs and MPCs are included due to confusion in terminology.