Comparison of the Direct Effects of Human Adipose- and Bone-Marrow-Derived Stem Cells on Postischemic Cardiomyoblasts in an In Vitro Simulated Ischemia-Reperfusion Model

Figure 4

Metabolic activity measurement and LDH assay. (a) The metabolic activity measured 24 hours after the ischemia-reperfusion injury significantly decreased in the cells after the ischemic conditions compared to the control group, but the metabolic activity was enhanced with all the applied treatments (control: 0.858±0.021 AU; I-R model: 0.065±0.033 AU; ACM: 0.225±0.013 AU; hASC: 0.652±0.089 AU; hBMSC: 0.607±0.059 AU; 𝑛=3, ^∗𝑃<0.05, ^∗∗∗𝑃<0.001). (b) LDH levels in the cell culture supernatant were significantly lower when ACM, hASC, or hBMSC therapy was carried out (I-R model: 0.225±0.006 AU; ACM: 0.208±0.009 AU; hASC: 0.148±0.005 AU; hBMSC: 0.146±0.004 AU; 𝑛=3, ^∗𝑃<0.05, ^∗∗∗𝑃<0.001). The stem cell treated groups are not significantly different from each other and also not different from the control.

(a)

(b)