Hippocampal Neurogenesis and the Brain Repair Response to Brief Stereotaxic Insertion of a Microneedle

Figure 4

Astrocytosis in hippocampus indicated by GFAP immunoreactivity. Panels on the left ((a), (c), and (e)) depict the unlesioned control side; panels (b), (d), and (f) show the lesioned side. (a), (b)=GFAP (green channel); (c), (d)=BrdU (red channel); (e), (f) merged channels (GFAP and BrdU). (g) Astrocytosis measured as extent of GFAP immunoreactivity (mean % area of field) was increased 6 times that of control (𝑛=3 mice at 2 wks; 3 mice at 4 wks; 6 sections per mouse). 2-way ANOVA showed that both treatment and time contribute significantly to the variance. Signal was significantly higher on lesioned side at 2 weeks. At 4 weeks, the mean signal had declined significantly. *𝑃<0.001; **𝑃<0.001 using Bonferroni correction for multiple comparisons.

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