Comparative Evaluation of Human Mesenchymal Stem Cells of Fetal (Wharton's Jelly) and Adult (Adipose Tissue) Origin during Prolonged In Vitro Expansion: Considerations for Cytotherapy
Figure 2
Variation in cell size (surface area in μm2) over time in culture and its association with proliferation kinetics. (a) Cell size (mean area ± SEM) as a phenotypic property of WJSC and ADSC during serial propagation. Dashed and long dashed horizontal lines denote mean cell size for ADSC (3876 μm2) and WJSC (2926 μm2), respectively; (b) regression analysis of ADSC size versus PDT at respective passages (, Spearman correlation coefficient = 0.915). Trendline equation and goodness of fit () are shown. Boxed data points correspond to ADSC with sizes over 3,880 μm2 (top 25% of cells); (c) regression analysis of WJSC size versus PDT at respective passages (, ). Open triangles, p.1–10; solid triangles, p.11–20. Trendline equation and goodness of fit are shown. Boxed data points correspond to WJSC with sizes over 4,600 μm2 (top 25%).
