Comparative Evaluation of Human Mesenchymal Stem Cells of Fetal (Wharton's Jelly) and Adult (Adipose Tissue) Origin during Prolonged In Vitro Expansion: Considerations for Cytotherapy

Table 2

Synopsis of proliferation kinetics data of WJSC and ADSC during long-term in vitro expansion.

	Mean PDT; (hrs)	Mean PD; per passage	Mean cell yield per passage (×10^6)	Theoretical total yield (×10^8) after five passages	Average time (days) required for obtaining; 80 × 10^6 cells
WJSC	31.9 ± 6.8 /; 38.4 ± 5.2*	2.9 ± 0.63 /; 2.8 ± 0.59	3.0 ± 0.82 /; 2.25 ± 0.8	120.5	17.8
ADSC	402.3 ± 215.1	0.77 ± 0.24	1.03 ± 0.35	1.03	34.8

WJSC data pairs in the first three columns correspond to cultures in the first 10 passages and in passages 11 to 20, respectively; ^∗𝑃<0.001. Values in the last two columns have been calculated on the basis of the assumption that all isolated cells are serially subcultivated with no intermediate freezing of cell stocks between successive passages.