Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation
Figure 3
Expression of Calcium-activated Potassium channels during formation of definitive endoderm. (a) Expression of SOX17 (green) after 5 days of DE differentiation. (b) Immunofluorescence analysis of SKCa proteins in DE cells. Indicated SKCa subtype (red). Scale bars as indicated. (c) Higher magnifications of indicated SKCa subtype (red). Scale bars as indicated. (d) Transcript levels of SK1 and SK2 remained relatively low during the DE differentiation. In contrast, mRNA levels of SK3 increased after 4 days of differentiation. SK4 mRNA levels slightly increased during the first days of differentiation and peaked on day 3 followed by a sharp decrease until day 5. Expression levels are shown relative to the housekeeping gene HMBS (, two different hiPSCs lines).
