Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC
Figure 6
Safranin red staining after chondrogenic differentiation of oMSC precultured in F10 or F2-S-EGF medium and subsequently differentiated following two different established protocols (TGF-3 or TGF-1/BMP-7) for 21 days. Proteoglycans in the extracellular matrix are stained in deep red. In 2 control conditions no pellet could be obtained during culture, indicating a low overall robustness of the pellets. . Scale bars 200 μm.
