Comparing the Gene Expression Profile of Stromal Cells from Human Cord Blood and Bone Marrow: Lack of the Typical “Bone” Signature in Cord Blood Cells
Figure 1
PrimeView human gene expression array. The gene expression of USSC (green), CBSC (blue), and BMSC (red, each in triplicate) was analyzed. (a) Venn-diagram illustrating common and unique expression of genes. The gene expression was filtered for each cell type: a probeset had to be expressed above the background (20th and 100th percentiles of the raw signal distribution) in at least two out of three replicates. This resulted in 39519 transcripts for the “group USSC,” 39493 transcripts for CBSC, and 39454 transcripts for BMSC which were compared. (b) principal component analysis (PCA) to depict the correlation of the single replicates of each cell type. Analyzed were those probesets that were expressed above the background in at least two out of three replicates in at least one out of three cell types which resulted in 40394 transcripts. The first three principal components accounted for 62.9% of the total variance (component 1 (-axis): 31.6%; component 2 (-axis): 18.3%; component 3 (-axis): 13.0%).
