Human Mesenchymal Stem Cells Display Reduced Expression of CD105 after Culture in Serum-Free Medium

Table 1

Antibodies for analytical fluorescence-activated cell sorting.

Antibody/dye	Conjugate	Company	Volume antibody*	Isotype	Company	Volume isotype*
CD29	APC	BD Biosciences	20 𝜇L	mouse IgG1 𝜅	BD Biosciences	20 𝜇L
CD44	PerCP-Cy5.5	BD Biosciences	5 𝜇L	mouse IgG2b 𝜅	BD Biosciences	20 𝜇L
CD45	V500	BD Biosciences	5 𝜇L	mouse IgG1 𝜅	BD Biosciences	5 𝜇L
CD73	PE	BD Biosciences	20 𝜇L	mouse IgG1 𝜅	BD Biosciences	20 𝜇L
CD90-biotin	Streptavidin-V450	BD Biosciences	3 𝜇L	mouse IgG1 𝜅	BD Biosciences	21 𝜇L
CD105	Alexa Fluor 488	AbD Serotec	10 𝜇L	mouse IgG1	AbD Serotec	10 𝜇L
NearIR (live/dead)	—	Invitrogen	0.5 𝜇L	—	—	—

∗Volumes required for up to 500,000 cells in 100 𝜇L.
APC (Allophycocyanin), PerCP-Cy5.5 (Peridinin chlorophyll protein-cyanine 5.5 conjugate), V500 (BD Horizon V500), PE (phycoerythrin), V450 (BD Horizon V450), NearIR (LIVE/DEAD Fixable Near-IR Dead Cell Stain).