Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique
Figure 3
Adipogenic differentiation of hMSCs in aggregate culture at two weeks, illustrating the loss of adipogenic differentiation potential with serial expansion in culture. The cells in both Figures are from the same donor preparation and were expanded using the identical medium formulation. (a): 2nd passage cells; (b): 10th passage cells. On average, passage 10 aggregates had slightly less than half the Oil Red positive area of passage 2 aggregates (, 2 donor preparations, 100 40x fields were measured) frozen section, Oil red-O with haematoxylin counterstain. Original magnification: 40x, scale bar: 50 μm.
