The Assessment of Parameters Affecting the Quality of Cord Blood by the Appliance of the Annexin V Staining Method and Correlation with CFU Assays
Figure 5
The reproducibility of ClonE for different samples is dependent on seeding density. When less than 100 CD3 cells were seeded per mL, the resulting ratios were extraordinarily high and varied stronger than those with higher seeding density. Only in the range from 100 to 200 CD34+/mL, constant ratios were observed close to the theoretically expected value of 1 : 1 (dotted line), while higher densities lead to lower ClonE values, probably due to limiting effects as well as impaired countability.
