The Assessment of Parameters Affecting the Quality of Cord Blood by the Appliance of the Annexin V Staining Method and Correlation with CFU Assays
Figure 6
(a) Total leukocytes showed lower viability when stored at room temperature in comparison to 4°C. (b) Regarding CD3 cells, results revealed no difference between storage at , room temperature and ; only storage at did drastically increase amount of nonviable cells (exemplarily shown in CB1; red triangles), making the according samples unsuitable for flow cytometric analysis after more than 60 hours.