Wnt1 Accelerates an Ex Vivo Expansion of Human Cord Blood CD34⁺CD38⁻ Cells

Figure 1

Proliferation of expanded CB cells. CB-CD34⁺ were maintained in 4 different cultures media: 2 serum-containing media (4F cIMDM, 5F cIMDM) and 2 serum-free media (4F KSR, 5F KSR). (a) Proliferation assay, (b) growth profile of total CD34⁺CD38⁻ cells, (c) CD133⁺CD38⁻ cells, and (d) CD34⁻CD38⁺ cells. After culturing the cells for 5 and 7 days, cells were harvested for analysis and normalized with day 0 (𝑛=3). *𝑃<0.001, **𝑃<0.02, and ***𝑃<0.05.

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