Research Article
Wnt1 Accelerates an Ex Vivo Expansion of Human Cord Blood CD34+CD38− Cells
Figure 4
Hematopoietic colony-forming units and differentiation capacity of expanded CB-CD34+ cells in vitro. (a) Colony-forming assay of 5-day-enriched CB-CD34+ cells in 4 different culture conditions (mean ± SD; ). (b) Photographs of CFU-GEMM, CFU-GM, BFU-E, CFU-G, and CFU-M after incubated in methylcellulose for 14 days. Liquid differentiation assay of 5-day-expanded CB-CD34+ cells from (c) 4F KSR and (d) 5F KSR conditions. In (c) and (d), (A) fresh CB-CD34+ cells, (B) 5-day-expanded CB-CD34+ cells, (C) 7-day-expanded CB-CD34+ cells, (D) erythroid lineage differentiation (EPO+SCF), (E) megakaryocytes differentiation (TPO+SCF), (F) lymphoid lineage differentiation (IL-7, Flt3-L, SCF+OP9), (G) granulocytes and macrophage lineages differentiation (GM-CSF+IL-3), and (H) mast cells and granulocyte lineages differentiation (SCF+IL-3). Bar correspondences as 10 μm.
