Wnt1 Accelerates an Ex Vivo Expansion of Human Cord Blood CD34+CD38− Cells
Table 2
Folds expansion of CD34+CD38−, CD133+CD38−, and CD34−CD38+ cells under various cytokine culture conditions.
Culture conditions
Day
Fold expansion
Name
Medium
Cytokines
CD34+CD38−
CD133+CD38−
CD34−CD38+
4F cIMDM
IMDM + FBS
Flt3-L, SCF, TPO, and IL-6
5
2.6 ± 1.1
0.7 ± 0.4
12.0 ± 1.4
7
4.2 ± 1.3
3.1 ± 1.6
62.8 ± 5.0
5F cIMDM
IMDM + FBS
Flt3-L, SCF, TPO, IL-6, and Wnt1
5
3.9 ± 1.0
2.5 ± 1.2
9.3 ± 1.3
7
6.6 ± 1.4
6.0 ± 2.6
89.7 ± 12.2***
4F KSR
IMDM + KSR
Flt3-L, SCF, TPO, and IL-6
5
6.3 ± 1.6
4.7 ± 2.0
7.6 ± 2.2
7
18.5 ± 0.4*
11.5 ± 3.4***
20.1 ± 6.8*
5F KSR
IMDM + KSR
Flt3-L, SCF, TPO, IL-6, and Wnt1
5
5.9 ± 1.2
5.4 ± 2.2
8.4 ± 2.2
7
24.3 ± 2.1*,**
12.3 ± 4.0***
13.0 ± 3.7*
Data represented as mean fold increasing in expansion of three independent CB sample ± SD expanded for 5 and 7 days subtracted with day 0 (). * versus 4F cIMDM and 5F cIMDM, ** versus 4F KSR, *** versus 4F cIMDM on the same day of culture.
