Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
Figure 3
Rhodamine-phalloidin and lectin histochemistry. (a) After 11 days of cultivation, fluorescence microscopy showed capillary tubes that are stained with rhodamine-phalloidin for F-actin (purple-red) and 4′,6-diamidino-2-phenylindole (DAPI) nucleic acid stain (blue). The asterisk () shows the mouse aortic explant. Scale bar = 20 μm. (b) After 11 days of cultivation, the capillary tubes are strongly positive for FITC-conjugated endothelial-cell-specific tomato lectin staining (yellowish-green) and DAPI nucleic acid stain (blue). The asterisk () shows the aortic explant. Scale bar = 20 μm.
