- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Stem Cells International
Volume 2013 (2013), Article ID 973508, 12 pages
Impact of Lipid Nutrition on Neural Stem/Progenitor Cells
1Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan
2Japan Society for the Promotion of Science, 8 Ichiban-cho, Chiyoda-ku, Tokyo 102-8472, Japan
Received 28 December 2012; Accepted 9 September 2013
Academic Editor: Kenneth Boheler
Copyright © 2013 Nobuyuki Sakayori et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The neural system originates from neural stem/progenitor cells (NSPCs). Embryonic NSPCs first proliferate to increase their numbers and then produce neurons and glial cells that compose the complex neural circuits in the brain. New neurons are continually produced even after birth from adult NSPCs in the inner wall of the lateral ventricle and in the hippocampal dentate gyrus. These adult-born neurons are involved in various brain functions, including olfaction-related functions, learning and memory, pattern separation, and mood control. NSPCs are regulated by various intrinsic and extrinsic factors. Diet is one of such important extrinsic factors. Of dietary nutrients, lipids are important because they constitute the cell membrane, are a source of energy, and function as signaling molecules. Metabolites of some lipids can be strong lipid mediators that also regulate various biological activities. Recent findings have revealed that lipids are important regulators of both embryonic and adult NSPCs. We and other groups have shown that lipid signals including fat, fatty acids, their metabolites and intracellular carriers, cholesterol, and vitamins affect proliferation and differentiation of embryonic and adult NSPCs. A better understanding of the NSPCs regulation by lipids may provide important insight into the neural development and brain function.
Neural stem/progenitor cells (NSPCs) are a specific population of cells in nervous system that has self-renew capacity and multipotency. In early brain development, NSPCs divide symmetrically and increase their number to produce sufficient NSPC pool. Subsequently, an NSPC divides asymmetrically to produce one NSPC and one differentiated cell in an orderly fashion . NSPCs at the early developmental stage generate a large amount of neurons, whereas those at the late developmental stage generate mainly glial cells . New neurons migrate out and form synapses with other neurons, establishing neuronal networks, which are supported by glial cells including astrocytes and oligodendrocytes. The fact that all neurons and glial cells consisting the adult nervous system originate from NSPCs shows no doubt about the importance of these NSPCs in brain development.
Neurogenesis was traditionally considered to finish just after birth, although the possibility of neurogenesis in the adult rat brain is suggested already in 1960s [3, 4]. After a few decades of doubt against adult neurogenesis in mammals, Reynolds and Weiss found that cells dissociated from adult mouse brains proliferate to form spherical balls in culture . These spherical balls are called “neurospheres” and are positive for nestin, a marker for NSPCs . Neurospheres can differentiate into neurons, astrocytes, and oligodendrocytes following the withdrawal of growth factors from the culture medium. Clonal culture eventually confirmed self-renewal capacity and multipotency of these spheres . When cells from a tissue form spheres in vitro, the original tissue is retrospectively considered to contain NSPCs. This selective culture of NSPCs for forming neurospheres is widely used in NSPC studies. Regarding in vivo studies, NSPCs are found as proliferating cells in the inner wall, subventricular zone (SVZ), of the lateral ventricle [8, 9]. It is further proven that cells in the SVZ that have features similar to astrocytes are NSPCs . Regarding hippocampal neurogenesis, proliferating cells  and immature neurons  exist in the subgranular zone (SGZ) of the adult dentate gyrus. Late 90s is an epoch-making period that multiple papers are published from various laboratories showing the existence of actively proliferating NSPCs in the SGZ in rats  and mice , of adult-born neurons in the monkey brain  and in the postmortem human brain . From these lines of evidence, it is now widely believed that NSPCs exist not only in the embryonic brain but also at least in two areas of the adult brain: the SVZ of the lateral ventricle and the SGZ of the dentate gyrus in the hippocampus. The NSPCs in the adult brain continuously produce new neurons that have important roles in rodent behaviors (see below), suggesting their significance in brain function.
Lipids are an important nutritional composition because they have high calorific value, compose biological structures, and produce biologically active substances. Lipid is an ambiguous term, and there is no definition widely accepted. It is frequently defined as naturally occurring compounds that are insoluble in water but soluble in nonpolar solvents. However, such a definition is somewhat misleading because many substances that are regarded as lipids are soluble in both water and nonpolar solvents. Lipids are often categorized into simple lipid, compound (or complex) lipid, and derived lipid , although another categorization is also well accepted . Simple lipid is an ester of fatty acids and alcohol, for example, fat and wax. Fat is stored in adipocytes and is believed to be used as energy source for all organs except for nervous system. Compound lipid is a lipid with more groups, including phosphoric acid or carbohydrate, for example, phospholipid and glycolipid, respectively. These compound lipids are components of cell membrane. Simple lipids and compound lipids are metabolized or hydrolyzed into derived lipids, for example, fatty acids, steroids, and fat-soluble vitamins. These derived lipids have strong bioactivity and regulate various biological functions.
Fatty acids are one of the derived lipids and behave as signaling molecules, precursors to families of lipid mediators, and components of both simple and compound lipids. In the brain, fatty acids are the major structural components; it is estimated that half of the neuronal membrane is composed of fatty acids [19–21]. Among fatty acids, long-chain polyunsaturated fatty acids (PUFAs), which have more than 16 carbon atoms and more than one cis double bond, have been implicated as critical nutritional factors for proper neural development and function [22–24]. Because the physiological properties of PUFAs largely depend on the position of the first double bond from the terminal methyl group of the carbon chain, PUFAs are categorized into n-3, n-6, and n-9 PUFAs by its position. They have the first double bond existing as the third, sixth, and ninth carbon-carbon bond from the methyl group, respectively. Most of lipids are synthesized de novo in mammals, while these n-3 PUFAs and n-6 PUFAs are not synthesized and must be obtained from diets . Thus, n-3 PUFAs, including α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), together with n-6 PUFAs, including linoleic acid (LA) and arachidonic acid (ARA), are referred to as essential fatty acids. In a more rigorous definition, essential fatty acids are ALA and LA. This is because DHA and ARA can be synthesized from ALA through EPA and from LA, respectively (Figure 1). However, we should keep in mind that the synthesis of DHA from its original precursor, ALA, is very scarce in human . The major end product of n-3 pathway is DHA, whereas that of the n-6 pathway in mammals is ARA. Actually, PUFAs in membrane phospholipids are mainly composed of DHA and ARA . These fatty acids have indispensable roles in various biological functions.
Brain contains a large amount of lipids because neurons have very complicated dendrites and long axons that are ensheathed by the cell membrane of oligodendrocytes. There are many pieces of literature showing that lipids play pivotal roles in neural development and brain function because lipid is included in diet and affects as an extrinsic factor . In this review, we focus on the effects of lipid nutrition in embryonic and adult NSPCs, mainly in rodents.
2. NSPCs and Their Function
2.1. Embryonic NSPCs in the Telencephalon
All neurons except for granule cells in the dentate gyrus and interneurons in the olfactory bulb (see below) are produced from embryonic NSPCs. All regions of the embryonic brain have NSPCs, and each character is slightly different. Here, we focus on embryonic NSPCs in the mouse telencephalon.
During early neural development, NSPCs emerge in the neural tissue at embryonic day (E) 8 in the mouse (or E10 in the rat). At this stage, NSPCs proliferate to expand the pool of NSPCs. Approximately at E10.5, NSPCs that reside in the inner wall of the neural tube start to produce cortical neurons. This region where NSPCs reside is termed the ventricular zone (VZ), and these NPSCs are called radial glial (RG) cells because their processes locate radially within the cortical primordium, and these cells exhibit astroglial properties . RG cells also produce basal progenitor cells that further proliferate in the subventricular zone (SVZ) neighboring the VZ [29–31]. Neurons are produced by direct neurogenesis from RG cells and by indirect neurogenesis from basal progenitor cells . Recently, another subtype of progenitor cells has been reported in the embryonic cortex. These progenitor cells are called outer radial glial (oRG) cells. oRG cells are generated directly from RG cells, form the outer subventricular zone (OSVZ), and produce neurons directly [33, 34]. oRG cells are implicated as an important source for cortical evolution because a recent study suggests that the development of oRG cells may be the cellular mechanism underlying expansion in primate corticogenesis . The initial neurons produced from RG cells form the preplate, which is subsequently divided into the subplate and the marginal zone. The marginal zone will form layer 1 of the neocortex. From E11.5 to 17.5, RG cells, basal progenitor cells, and oRG cells produce projection neurons of the different neocortical layers in a strictly controlled temporal order, from layer 6 to layer 2/3 (Figure 2) [36, 37], although a recent report has shown that neuronal progenitor cells that will differentiate into upper layer neurons (layers 2–4) are already produced even in early neural development . These neurons develop the cortical plate, which will give rise to the major layers (layers 2–6) of the gray matter of the neocortex, sandwiched by the subplate and the marginal zone. The production of cortical projection neurons is completed by the end of the embryonic period.
During late neural development, astrocytes are differentiated from NSPCs, and their production has its peak just after birth. Thus, there is a transition from neurogenic to gliogenic in the character of NSPCs. This transition is well studied in vitro because cultured NSPCs recapitulate the transition. NSPCs cultured for a short period differentiate into neurons, whereas those cultured for a long period produce more glial cells . Moreover, the neurogenic-to-gliogenic fate switch of NSPCs can be observed in culture in clones of single NSPCs . Several molecular mechanisms for the initiation/inhibition of astrocyte differentiation from NSPCs have been proposed [39–42].
Oligodendrocytes in the cortex are produced in three waves: the first and second waves occur in the embryonic ventral telencephalon, and the third wave occurs among postnatal cortical progenitors . During embryonic development, oligodendrocyte precursor cells (OPCs) are thought to be generated from NSPCs located in the ventral telencephalon [43–45]. OPCs then migrate tangentially into the developing cortex [46, 47]. In addition to the production of embryonic OPCs, a postnatal wave of OPCs has been reported in the cortical SVZ [48, 49]. It is thought that a large portion of oligodendrocytes in the adult cortex originates from these OPCs . OPCs differentiate into oligodendrocytes that form the myelin sheath surrounding neuronal axons. In some regions of the healthy adult brain, approximately 60% of OPCs continue to proliferate to generate oligodendrocytes . Therefore, oligodendrogenesis is important throughout life.
2.2. Adult NSPCs in the SVZ of the Lateral Ventricle
Several cell types are involved in adult neurogenesis in the SVZ (Figure 3). A lineage tracing study and fate-mapping study have revealed that GFAP-expressing cells that have morphological features similar to RG cells serve as quiescent neural stem cells [10, 52]. GFAP-expressing radial glia-like cells are referred to as type B cells . These cells extend their small apical process that retains primary cilium to the ventricle. In addition, their basal processes reach blood vessels and form endfeet [53, 54], suggesting that type B cells are directly regulated by both cerebrospinal fluid and bloodstream. Type B cells give rise to actively proliferating progenitors, referred to as type C cells [10, 55]. Immature neuroblasts called type A cells are generated from type C cells and migrate a long way to the olfactory bulb (OB) through the rostral migratory stream (RMS) [8, 56]. Once type A cells reach the core of the olfactory bulb, they separate from the RMS and migrate radially toward the surface of the OB. Most of the type A cells become GABAergic granule neurons, but a minority of them become GABAergic/dopaminergic periglomerular neurons [57, 58].
Although the potential function of adult-born neurons in the OB is still under investigation, cumulative evidence has indicated their important roles in the OB functions. Half of the adult-born neurons in the OB are incorporated into the preexisting neural circuitry , and genetic ablation of newly generated cells in the SVZ resulted in a significant reduction in the number of mature granule neurons in the OB . Many experiments have addressed the functional importance of adult-born neurons in olfactory-related behaviors. Although adult-born neurons in the OB are not required for the discrimination between similar chemical odors and response to innate aversive odor such as fox scent [60–62], they are required for olfactory-fear conditioning , olfactory perceptual learning , and long-term olfactory memory . A recent study has demonstrated that adult-born neurons affect the response of mice to innate aversive odor when associated with reward . Thus, adult-born neurons in the OB are important in olfaction-dependent behaviors via long-term structural integration.
2.3. Adult NSPCs in the SGZ of the Dentate Gyrus in the Hippocampus
The production and maturation of new granule neurons in the SGZ occur in a sequential manner as well as those in the SVZ (Figure 3). In the SGZ, GFAP-expressing radial glia-like cells are referred to as type 1 cells . Type 1 cells extend their long apical process into the molecular layer (ML) of the dentate gyrus [52, 67], and their basal processes contact with blood vessels in the same fashion as type B cells in the SVZ . Type 1 cells make a transition to fast proliferating intermediate progenitors called type 2 cells, which in turn generate neuroblasts (type 3 cells) . Type 3 cells become immature neurons and migrate a short way into the inner granule cell layer (GCL), where they differentiate into granule neurons. Within two weeks, newborn granule neurons extend their dendrites toward ML and project their axon (mossy fiber) to CA3 pyramidal neurons through the hilus of dentate gyrus . Compared to mature neurons, newborn granule neurons have hyperexcitability and enhanced synaptic plasticity during a certain period of time, contributing to shaping the existing circuit in response to external stimuli [71–73]. Newborn granule neurons gradually mature and work within preexisting neural circuit.
Adult neurogenesis in the SGZ has been implicated in several hippocampus-dependent behaviors. It has important roles in hippocampus-dependent learning and memory. Morris water maze task that is considered to activate the hippocampal neural circuit enhances the survival of newborn neuron in the dentate gyrus . Irradiation or genetic manipulation of newborn neurons has also shown that adult neurogenesis in the SGZ is required for short- or long-term spatial memory [60, 75–77]. In addition to learning and memory, adult neurogenesis in the SGZ is also required for the formation of contextual fear memory and transition of such a kind of memory from the hippocampus to higher brain regions [78, 79]. Furthermore, other groups demonstrated that adult newborn neurons in dentate gyrus also contribute to the pattern separation by ablation with irradiation of adult neurogenesis in the SGZ  and by selectively inhibiting the synaptic transmission of old granule cells in dentate gyrus . Pattern separation is a process to discriminate similar but distinct matters, and it is thought that the dentate gyrus and CA3 of the hippocampus play an important role in this process [82, 83]. Adult neurogenesis in the SGZ has also been implicated in mood control. Patients with major depressive disorder exhibit a reduced hippocampal volume, suggesting that decreased neurogenesis is one of the contributing factors . In fact, antidepressant treatment in rodents and nonhuman primates increases neurogenesis in the dentate gyrus, and ablation of newborn neurons by irradiation attenuates the efficacy of antidepressant such as imipramine and fluoxetine on behavior [85–87]. Although there is criticism regarding the association between depression and neurogenesis (reviewed by Petrik et al. ), a recent study indicated that newborn neurons in the dentate gyrus are required for buffering the stress response through hypothalamo-pituitary-adrenal axis (HPA-axis) . It seems that neurogenesis in the adolescent stage may contribute to the establishment of sensorimotor gating in the rat  and in the mouse (our unpublished results). Thus, various hippocampal functions are indeed at least in part related to newborn neurons in the SGZ.
Adult neurogenesis in the SGZ is well understood because it is regulated by lots of physiological stimulations. Physical exercise such as voluntary running enhances cell proliferation in the SGZ, and enriched environment promotes the survival of newborn neurons [14, 91]. On the other hand, various stress paradigms, for example, subordination, resident intruder, restraint, and isolation stresses, decrease cell proliferation in the SGZ [15, 92–94]. Aging also decreases in cell proliferation and neuronal differentiation in the SGZ . A recent study has shown that age-associated decline of neurogenesis in the SGZ is attributable to depletion of neural stem cells followed by their differentiation into astrocyte . Further study will reveal flexible characters of adult NSPCs in the SGZ and provide us with an insight into the regulation of stem cell activity in the adult tissue.
3. Modulation of NSPCs by Lipid Nutrition
NSPCs are regulated by various intrinsic and extrinsic factors. Intrinsic factors including genetic networks are difficult to manipulate. However, diet is one of the important extrinsic factors that can be easily manipulated. Here, we review nutritional effects of lipids on NSPCs.
Fat is a major dietary source for lipids and has significant involvements in NSPCs. Dietary fat is called triglyceride because it is a triester of glycerol and fatty acids. In triglyceride form, fat cannot be absorbed by the intestines. Pancreatic lipase hydrolyses the ester bond and releases fatty acids from glycerol. These derivatives can be absorbed and used by various organs. It is reported that obesity-inducing high-fat diet (HFD), when administered to mother mice, impaired the proliferation of early postnatal NSPCs but not of embryonic and young-adult NSPCs, in the hippocampus of their offspring . Interestingly, another report has shown that HFD caused impairment of the proliferation of adult NSPCs in the SGZ without causing the apparent obesity . These studies suggest the possibility that excess intake of fat is detrimental in NSPCs.
3.2. n-3 and n-6 PUFAs
Various in vitro studies have shown that n-3 and n-6 PUFAs are involved in the regulation of NSPCs. Previously, we have shown that DHA and ARA affect proliferation and differentiation of embryonic NSPCs . We assayed embryonic NSPCs by neurosphere culture in DHA/ARA-free medium with/without DHA or ARA. For neurogenic NSPCs, DHA and ARA promoted the maintenance of NSPCs, but no detectable effects on differentiation were observed. For gliogenic NSPCs, DHA promoted the maintenance and neuronal differentiation of gliogenic NSPCs. Conversely, ARA did not promote the maintenance of NSPCs but promoted differentiation into astrocytes. We also confirmed that higher concentration of DHA had more toxic effects on the survival of NSPCs compared with that of ARA. This makes sense because DHA has more double bonds than ARA, and lipid peroxidation is a form of oxidative stress, which is toxic to cells and dampens cell survival . These results show that DHA and ARA directly regulate embryonic NSPCs and that the effects of DHA and ARA on embryonic NSPCs depend on the stage of development. Other groups have also shown that DHA promotes the proliferation and neuronal differentiation of cultured NSPCs generated from embryonic stem (ES) cells  and that DHA induces the neuronal differentiation of cultured embryonic NSPCs [101–103]. Kan et al. found that both DHA and ARA are necessary for the neuronal differentiation from mesenchymal stem cells , suggesting that ARA may also be necessary for neuronal differentiation under some conditions.
The precursors of DHA and ARA, that is, ALA and LA, also affect NSPCs in vitro. We have previously shown that ALA and LA promote the maintenance of embryonic NSPCs . On the other hand, it is also reported that conjugated linoleic acid (CLA), a positional and geometrical isomer of LA, promotes the neuronal differentiation of embryonic NSPCs, while LA has no such effect . DHA and ARA may be synthesized in these experiments because embryonic NSPCs express enzymes that are necessary for the synthesis of DHA and ARA from ALA and LA . It is possible that these enzymes regulate the metabolism of n-3 and n-6 PUFAs in the developing brain to regulate proliferation and differentiation of embryonic NSPCs.
n-3 and n-6 PUFAs actually affect NSPCs in vivo. We have previously shown that, by feeding DHA-rich diet to mother rats, there were no detectable effects on the proliferation of postnatal NSPCs in the SGZ of their offspring. However, it is reported that DHA is actually incorporated into the brain of offspring via the mother’s breast milk , and another group has found that oral administration of DHA promoted adult neurogenesis in the hippocampus of rats fed with a fish oil-deficient diet over three generations . It is also reported that by feeding n-3 PUFAs-rich diet to aged rat, immature neurons in the dentate gyrus was increased . This is because age-related decrease of phospholipids  may partially be compensated by feeding n-3 PUFAs-rich diet. It is also known that feeding an n-3 PUFAs-deficient diet to pregnant rats causes inhibition or delay of neurogenesis in the embryonic brains of pups . Regarding the effects of ARA, we have previously shown that supplementation of ARA to rat pups through mother’s breast milk by feeding ARA-rich diet to mother rats promotes the proliferation of postnatal NSPCs in the SGZ . These data suggest that DHA is necessary but not sufficient for regulating NSPCs in physiological condition but that ARA is sufficient to affect NSPCs even in the physiological condition.
3.3. Metabolites of n-6 PUFAs
Like n-6 PUFAs, their metabolites also influence NSPCs. n-6 PUFAs are metabolized into various substances [105, 110], including prostaglandins (PGs). PGs are strong lipid mediators and are known to have various functions in the regulation of NSPCs. E-type prostaglandin 2 (PGE2) is synthesized from ARA by cyclooxygenases (COXs) and microsomal PGE synthase-1 and functions by binding to PGE2 receptors, EP1 to EP4. EP3 is expressed in adult NSPCs [111, 112], and an EP3 agonist promotes the proliferation of adult NSPCs in the SGZ . D-type prostaglandin 2 (PGD2) is also synthesized from ARA by COXs and two types of PGD synthase and is nonenzymatically metabolized into 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), which also promoted the proliferation of cultured embryonic NSPCs and postnatal NSPCs in the hippocampus . The fact that PGD2 is the most abundant PG in the brain  suggests the importance of 15d-PGJ2 function in NSPCs. Thus, mediators derived from ARA have significant roles in the regulation of NSPCs.
3.4. Fatty Acid Binding Proteins
Fatty acids taken from diets are delivered to various organs, but fatty acids need to be bound to proteins within the aqueous cytoplasm and blood plasma. This is because the solubility of fatty acids in aqueous solution is extremely low. Albumin can facilitate PUFA transport in blood plasma , and fatty acid binding proteins (Fabps) are intracellular carriers that accommodate PUFAs . Among Fabps, Fabp3 (H-Fabp), Fabp5 (E-Fabp, K-Fabp, or S-Fabp) and Fabp7 (BLBP or B-Fabp) are the members expressed in the brain. Fabp3 is not expressed in the embryonic brain but appears in the adult brain . Fabp5 is expressed in NSPCs in the embryonic brain and in the SGZ of the dentate gyrus in the hippocampus as well as in neurons in the cerebral cortex and in astrocytes [119–121]. Fabp7 is also expressed in NSPCs located in the VZ of the embryonic brain and in the SGZ of the dentate gyrus in the hippocampus and in astrocytes [121–124]. Among these Fabps, Fabp3 and Fabp5 bind to ARA [125, 126], while Fabp5 and more preferentially Fabp7 bind to DHA [127–129]. Due to these multiple Fabps, neural cells have access to various types of PUFAs at an adequate level.
The function of Fabps in the NSPCs has been studied by analyzing genetically altered mice. Fabp3 is not expressed in the embryonic brain and in the adult NSPCs , suggesting no function in NSPCs. Fabp5 and Fabp7 are strongly expressed in the embryonic brain, but no detectable abnormalities are reported in the gross anatomy of the brain of Fabp5 and Fabp7 KO mice [120, 130]. However, we have previously reported that the proliferation of postnatal NSPCs in the SGZ was decreased in both Fabp5 and Fabp7 KO mice [121, 124]. More severely reduced proliferation of NSPCs in the SGZ is observed in Fabp5/7 double KO mice . In addition, acute knockdown of Fabp7 promotes precocious neuronal differentiation, suggesting that Fabp7 is necessary for the maintenance of NSPCs . These data show that Fabps, the intracellular carriers of PUFAs, have important roles in NSPCs.
Cholesterol is one of the well-studied steroids in nutritional research. Cholesterol is an essential structural component of the cell membrane and forms lipid rafts interacting with various proteins to generate specific cholesterol-based membrane microdomains. These domains are important in membrane traffic and signal transduction . Cholesterol also serves as a precursor for the steroid hormones and bile acids and is also a component of lipoproteins, that is, carriers for various lipids. About 25% of unesterified cholesterol is concentrated in the CNS ; the brain and spinal cord are the organs that contain the most abundant cholesterol among all organs, suggesting their importance in neural functions.
Roles of cholesterol in the regulation of NSPCs are poorly understood despite its significant functions on synaptogenesis . The amount of cholesterol dramatically increases during cortical development , and an apical plasma membrane protein, prominin-1, of embryonic NSPCs in the VZ directly interacts with membrane cholesterol , suggesting that cholesterol has important roles in neural development. Conditional ablation of cholesterol biosynthesis in embryonic NSPCs leads to angiogenesis by increased vascular endothelial growth factor (VEGF) expression in embryonic NSPCs . This may be a mechanism to compensate for the ablation of endogenous cholesterol, suggesting that cholesterol has essential roles in NSPCs. Regarding the roles of exogenous cholesterol, it is reported that the proliferation of adult NSPCs in the SGZ is decreased followed by feeding a high-cholesterol diet without increasing calorie intake . These data suggest that appropriate biosynthesis/intake of cholesterol is necessary for the integrity of NSPCs.
3.6. Fat-Soluble Vitamins
Fat-soluble vitamins including vitamin A and E are important nutrients and also regulate the conditions of NSPCs. Vitamin A is well known to be necessary for visual functions and regulation of some genes including αB-crystallin and fibroblast growth factor 8 . Regarding their effects on NSPCs, it is reported that the injection of an excess dose of retinoic acid (RA), an active form of vitamin A, to mice significantly reduced the proliferation of adult NSPCs in the SGZ and SVZ, suppressed adult hippocampal neurogenesis, and disrupted the ability to perform a spatial radial maze task . Depletion of RA in adult mice, on the other hand, leads to significantly decreased neuronal differentiation and reduced neuronal survival within the granular cell layer of the dentate gyrus . RA can restore adult hippocampal neurogenesis in retinoid-deficient rats . These data suggest that a suitable dose of RA is essential for NSPCs.
Vitamin E is a group of compounds with well-known antioxidant functions. Supplementation of α-tocopherol, the most important compound of vitamin E, inhibits the proliferation of adult NSPCs in SGZ, conversely promoting neurogenesis and enhancing the neuronal survival in the dentate gyrus . On the contrary, vitamin E deficiency in rats causes increased proliferation of adult NSPCs in SGZ and reduced neuronal survival . These data clearly show that vitamin E promotes neuronal differentiation of NSPCs and survival of neurons.
3.7. Confounding Factors in Nutritional Research
Not only nutritional contents but also calorie intake, meal frequency, and meal hardness do affect proliferation and differentiation of NSPCs. Restriction of calorie intake increases the numbers of newly generated cells in the dentate gyrus of the hippocampus as a result of increased cell survival . Extending the time between meals without reducing calorie intake also increases adult hippocampal neurogenesis . In addition, cell proliferation in SGZ is decreased followed by feeding powder diet compared to by feeding solid diet . These parameters can be confounding factors that affect basal characters of NSPCs. There are more possible confounding factors that may affect NSPCs, including taste and smell of food, because these factors play important roles in regulating food intake. Researchers on nutritional studies should keep in mind these potential secondary effects.
Embryonic NSPCs are essential for neural development and adult NSPCs are important for various neural functions, including cognition and mood. It is now becoming clearer that lipid nutrition has a significant impact on neural development and brain functions. Modulating proliferation and differentiation of NSPCs by diet could be an easily controllable intervention that may prevent neurodevelopmental disorders, cognitive decline during aging, and various kinds of psychiatric disorders. Indeed, n-3 PUFAs have ameliorative/preventive effects on patients with schizophrenia [148–151], mood disorders [152–154], and posttraumatic stress disorder [155–157]. A recent report has shown that ARA may potentially have a therapeutic effect on autistic patients . Although effects of lipid nutrition are well focused, mechanisms by which lipid nutrition modulates NSPCs are poorly understood. Fatty acids serve as ligands for several G-protein-coupled receptors. It is recently reported that one of such receptors, that is, GPR40, is necessary for DHA-inducing neuronal differentiation of embryonic NSPCs . GPR40-dependent phospholipase activation may thus be a possible signaling pathway of DHA. Further studies are necessary for comprehensive understanding of the effects of lipid nutrition.
Conflict of Interests
The authors have no potential conflict of interests.
The authors thank Drs. Takeo Yoshikawa, Yutaka Matsuoka, and Takahiro Moriya for critically reading the paper. This work was supported by the Research Fellowship of Japan Society for the Promotion of Science for Young Scientists given to N. Sakayori from the Japan Science and Technology Agency.
- S. Temple, “The development of neural stem cells,” Nature, vol. 414, no. 6859, pp. 112–117, 2001.
- X. Qian, Q. Shen, S. K. Goderie et al., “Timing of CNS cell generation: a programmed sequence of neuron and glial cell production from isolated murine cortical stem cells,” Neuron, vol. 28, no. 1, pp. 69–80, 2000.
- J. Altman, “Are new neurons formed in the brains of adult mammals?” Science, vol. 135, no. 3509, pp. 1127–1128, 1962.
- J. Altman, “Autoradiographic investigation of cell proliferation in the brains of rats and cats,” The Anatomical Record, vol. 145, pp. 573–591, 1963.
- B. A. Reynolds and S. Weiss, “Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system,” Science, vol. 255, no. 5052, pp. 1707–1710, 1992.
- U. Lendahl, L. B. Zimmerman, and R. D. McKay, “CNS stem cells express a new class of intermediate filament protein,” Cell, vol. 60, no. 4, pp. 585–595, 1990.
- B. A. Reynolds and S. Weiss, “Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell,” Developmental Biology, vol. 175, no. 1, pp. 1–13, 1996.
- M. B. Luskin, “Restricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone,” Neuron, vol. 11, no. 1, pp. 173–189, 1993.
- C. Lois and A. Alvarez-Buylla, “Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia,” Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 5, pp. 2074–2077, 1993.
- F. Doetsch, I. Caille, D. A. Lim, J. M. Garcia-Verdugo, and A. Alvarez-Buylla, “Subventricular zone astrocytes are neural stem cells in the adult mammalian brain,” Cell, vol. 97, no. 6, pp. 703–716, 1999.
- H. A. Cameron, C. S. Woolley, B. S. McEwen, and E. Gould, “Differentiation of newly born neurons and glia in the dentate gyrus of the adult rat,” Neuroscience, vol. 56, no. 2, pp. 337–344, 1993.
- T. Seki and Y. Arai, “Highly polysialylated neural cell adhesion molecule (NCAM-H) is expressed by newly generated granule cells in the dentate gyrus of the adult rat,” Journal of Neuroscience, vol. 13, no. 6, pp. 2351–2358, 1993.
- H. G. Kuhn, H. Dickinson-Anson, and F. H. Gage, “Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation,” Journal of Neuroscience, vol. 16, no. 6, pp. 2027–2033, 1996.
- G. Kempermann, H. G. Kuhn, and F. H. Gage, “More hippocampal neurons in adult mice living in an enriched environment,” Nature, vol. 386, no. 6624, pp. 493–495, 1997.
- E. Gould, P. Tanapat, B. S. Mcewen, G. Flügge, and E. Fuchs, “Proliferation of granule cell precursors in the dentate gyrus of adult monkeys is diminished by stress,” Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 6, pp. 3168–3171, 1998.
- P. S. Eriksson, E. Perfilieva, T. Björk-Eriksson et al., “Neurogenesis in the adult human hippocampus,” Nature Medicine, vol. 4, no. 11, pp. 1313–1317, 1998.
- W. R. Bloor, “Outline of a classification of the lipids,” Proceedings of the Society for Experimental Biology and Medicine, vol. 17, pp. 138–140, 1920.
- E. Fahy, S. Subramaniam, R. C. Murphy et al., “Update of the lipid maps comprehensive classification system for lipids,” Journal of Lipid Research, vol. 50, pp. S9–S14, 2009.
- M. Zerouga, F. Beauge, E. Niel, G. Durand, and J. M. Bourre, “Interactive effects of dietary (n-3) polyunsaturated fatty acids and chronic ethanol intoxication on synaptic membrane lipid composition and fluidity in rats,” Biochimica et Biophysica Acta, vol. 1086, no. 3, pp. 295–304, 1991.
- J. M. Bourre, M. Bonneil, J. Chaudiere et al., “Structural and functional importance of dietary polyunsaturated fatty acids in the nervous system,” Advances in Experimental Medicine and Biology, vol. 318, pp. 211–229, 1992.
- S. I. Rapoport, “In vivo approaches and rationale for quantifying kinetics and imaging brain lipid metabolic pathways,” Prostaglandins and Other Lipid Mediators, vol. 77, no. 1–4, pp. 185–196, 2005.
- N. Gordon, “Peroxisomal disorders,” Brain and Development, vol. 9, no. 6, pp. 571–575, 1987.
- N. Gordon, “Nutrition and cognitive function,” Brain and Development, vol. 19, no. 3, pp. 165–170, 1997.
- M. Hamosh and N. Salem Jr., “Long-chain polyunsaturated fatty acids,” Biology of the Neonate, vol. 74, no. 2, pp. 106–120, 1998.
- P. S. Sastry, “Lipids of nervous tissue: composition and metabolism,” Progress in Lipid Research, vol. 24, no. 2, pp. 69–176, 1985.
- J. T. Brenna, N. Salem Jr., A. J. Sinclair, and S. C. Cunnane, “α-linolenic acid supplementation and conversion to n-3 long-chain polyunsaturated fatty acids in humans,” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 80, no. 2-3, pp. 85–91, 2009.
- A. S. Ryan, J. D. Astwood, S. Gautier, C. N. Kuratko, E. B. Nelson, and N. Salem Jr., “Effects of long-chain polyunsaturated fatty acid supplementation on neurodevelopment in childhood: a review of human studies,” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 82, no. 4–6, pp. 305–314, 2010.
- M. Götz and W. B. Huttner, “The cell biology of neurogenesis,” Nature Reviews Molecular Cell Biology, vol. 6, no. 10, pp. 777–788, 2005.
- S. C. Noctor, V. Martinez-Cerdeño, L. Ivic, and A. R. Kriegstein, “Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases,” Nature Neuroscience, vol. 7, no. 2, pp. 136–144, 2004.
- W. Haubensak, A. Attardo, W. Denk, and W. B. Huttner, “Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 9, pp. 3196–3201, 2004.
- T. Miyata, A. Kawaguchi, K. Saito, M. Kawano, T. Muto, and M. Ogawa, “Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells,” Development, vol. 131, no. 13, pp. 3133–3145, 2004.
- R. F. Hevner, R. D. Hodge, R. A. Daza, and C. Englund, “Transcription factors in glutamatergic neurogenesis: conserved programs in neocortex, cerebellum, and adult hippocampus,” Neuroscience Research, vol. 55, no. 3, pp. 223–233, 2006.
- A. Shitamukai, D. Konno, and F. Matsuzaki, “Oblique radial glial divisions in the developing mouse neocortex induce self-renewing progenitors outside the germinal zone that resemble primate outer subventricular zone progenitors,” Journal of Neuroscience, vol. 31, no. 10, pp. 3683–3695, 2011.
- X. Wang, J. W. Tsai, B. Lamonica, and A. R. Kriegstein, “A new subtype of progenitor cell in the mouse embryonic neocortex,” Nature Neuroscience, vol. 14, no. 5, pp. 555–561, 2011.
- D. V. Hansen, J. H. Lui, P. R. Parker, and A. R. Kriegstein, “Neurogenic radial glia in the outer subventricular zone of human neocortex,” Nature, vol. 464, no. 7288, pp. 554–561, 2010.
- J. B. Angevine Jr. and R. L. Sidman, “Autoradiographic study of cell migration during histogenesis of cerebral cortex in the mouse,” Nature, vol. 192, no. 4804, pp. 766–768, 1961.
- V. S. Caviness Jr. and T. Takahashi, “Proliferative events in the cerebral ventricular zone,” Brain and Development, vol. 17, no. 3, pp. 159–163, 1995.
- S. J. Franco, C. Gil-Sanz, I. Martinez-Garay, et al., “Fate-restricted neural progenitors in the mammalian cerebral cortex,” Science, vol. 337, no. 6095, pp. 746–749, 2012.
- F. D. Miller and A. S. Gauthier, “Timing is everything: making neurons versus glia in the developing cortex,” Neuron, vol. 54, no. 3, pp. 357–369, 2007.
- H. Naka, S. Nakamura, T. Shimazaki, and H. Okano, “Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development,” Nature Neuroscience, vol. 11, no. 9, pp. 1014–1023, 2008.
- Y. Hirabayashi, N. Suzki, M. Tsuboi et al., “Polycomb limits the neurogenic competence of neural precursor cells to promote astrogenic fate transition,” Neuron, vol. 63, no. 5, pp. 600–613, 2009.
- Y. Kishi, Y. Fujii, Y. Hirabayashi, and Y. Gotoh, “HMGA regulates the global chromatin state and neurogenic potential in neocortical precursor cells,” Nature Neuroscience, vol. 15, no. 8, pp. 1127–1133, 2012.
- N. Kessaris, M. Fogarty, P. Iannarelli, M. Grist, M. Wegner, and W. D. Richardson, “Competing waves of oligodendrocytes in the forebrain and postnatal elimination of an embryonic lineage,” Nature Neuroscience, vol. 9, no. 2, pp. 173–179, 2006.
- W. He, C. Ingraham, L. Rising, S. Goderie, and S. Temple, “Multipotent stem cells from the mouse basal forebrain contribute GABAergic neurons and oligodendrocytes to the cerebral cortex during embryogenesis,” Journal of Neuroscience, vol. 21, no. 22, pp. 8854–8862, 2001.
- S. E. Ross, M. E. Greenberg, and C. D. Stiles, “Basic helix-loop-helix factors in cortical development,” Neuron, vol. 39, no. 1, pp. 13–25, 2003.
- J. L. Thomas, N. Spassky, E. M. Perez Villegas, et al., “Spatiotemporal development of oligodendrocytes in the embryonic brain,” Journal of Neuroscience Research, vol. 59, no. 4, pp. 471–476, 2000.
- N. Tekki-Kessaris, R. Woodruff, A. C. Hall et al., “Hedgehog-dependent oligodendrocyte lineage specification in the telencephalon,” Development, vol. 128, no. 13, pp. 2545–2554, 2001.
- A. Ivanova, E. Nakahira, T. Kagawa et al., “Evidence for a second wave of oligodendrogenesis in the postnatal cerebral cortex of the mouse,” Journal of Neuroscience Research, vol. 73, no. 5, pp. 581–592, 2003.
- C. A. Marshall, S. O. Suzuki, and J. E. Goldman, “Gliogenic and neurogenic progenitors of the subventricular zone: who are they, where did they come from, and where are they going?” Glia, vol. 43, no. 1, pp. 52–61, 2003.
- J. A. Gorski, T. Talley, M. Qiu, L. Puelles, J. L. Rubenstein, and K. R. Jones, “Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage,” Journal of Neuroscience, vol. 22, no. 15, pp. 6309–6314, 2002.
- L. E. Rivers, K. M. Young, M. Rizzi et al., “PDGFRA/NG2 glia generate myelinating oligodendrocytes and piriform projection neurons in adult mice,” Nature Neuroscience, vol. 11, no. 12, pp. 1392–1401, 2008.
- S. Fukuda, F. Kato, Y. Tozuka, M. Yamaguchi, Y. Miyamoto, and T. Hisatsune, “Two distinct subpopulations of nestin-positive cells in adult mouse dentate gyrus,” Journal of Neuroscience, vol. 23, no. 28, pp. 9357–9366, 2003.
- Z. Mirzadeh, F. T. Merkle, M. Soriano-Navarro, J. M. Garcia-Verdugo, and A. Alvarez-Buylla, “Neural stem cells confer unique pinwheel architecture to the ventricular surface in neurogenic regions of the adult brain,” Cell Stem Cell, vol. 3, no. 3, pp. 265–278, 2008.
- M. Tavazoie, L. van der Veken, V. Silva-Vargas et al., “A specialized vascular niche for adult neural stem cells,” Cell Stem Cell, vol. 3, no. 3, pp. 279–288, 2008.
- F. Doetsch, J. M. García-Verdugo, and A. Alvarez-Buylla, “Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain,” Journal of Neuroscience, vol. 17, no. 13, pp. 5046–5061, 1997.
- C. Lois, J. M. García-Verdugo, and A. Alvarez-Buylla, “Chain migration of neuronal precursors,” Science, vol. 271, no. 5251, pp. 978–981, 1996.
- M. A. Hack, A. Saghatelyan, A. de Chevigny et al., “Neuronal fate determinants of adult olfactory bulb neurogenesis,” Nature Neuroscience, vol. 8, no. 7, pp. 865–872, 2005.
- M. Kohwi, N. Osumi, J. L. Rubenstein, and A. Alvarez-Buylla, “Pax6 is required for making specific subpopulations of granule and periglomerular neurons in the olfactory bulb,” Journal of Neuroscience, vol. 25, no. 30, pp. 6997–7003, 2005.
- L. Petreanu and A. Alvarez-Buylla, “Maturation and death of adult-born olfactory bulb granule neurons: role of olfaction,” Journal of Neuroscience, vol. 22, no. 14, pp. 6106–6113, 2002.
- I. Imayoshi, M. Sakamoto, T. Ohtsuka et al., “Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain,” Nature Neuroscience, vol. 11, no. 10, pp. 1153–1161, 2008.
- F. Lazarini, M. A. Mouthon, G. Gheusi et al., “Cellular and behavioral effects of cranial irradiation of the subventricular zone in adult mice,” PLoS ONE, vol. 4, no. 9, Article ID e7017, 2009.
- M. T. Valley, T. R. Mullen, L. C. Schultz, B. T. Sagdullaev, and S. Firestein, “Ablation of mouse adult neurogenesis alters olfactory bulb structure and olfactory fear conditioning,” Frontiers in Neuroscience, vol. 3, article 51, 2009.
- M. M. Moreno, C. Linster, O. Escanilla, J. Sacquet, A. Didier, and N. Mandairon, “Olfactory perceptual learning requires adult neurogenesis,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 42, pp. 17980–17985, 2009.
- S. Sultan, N. Mandairon, F. Kermen, S. Garcia, J. Sacquet, and A. Didier, “Learning-dependent neurogenesis in the olfactory bulb determines long-term olfactory memory,” The FASEB Journal, vol. 24, no. 7, pp. 2355–2363, 2010.
- M. Sakamoto, I. Imayoshi, T. Ohtsuka, M. Yamaguchi, K. Mori, and R. Kageyama, “Continuous neurogenesis in the adult forebrain is required for innate olfactory responses,” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 20, pp. 8479–8484, 2011.
- V. Filippov, G. Kronenberg, T. Pivneva et al., “Subpopulation of nestin-expressing progenitor cells in the adult murine hippocampus shows electrophysiological and morphological characteristics of astrocytes,” Molecular and Cellular Neuroscience, vol. 23, no. 3, pp. 373–382, 2003.
- B. Seri, J. M. García-Verdugo, B. S. McEwen, and A. Alvarez-Buylla, “Astrocytes give rise to new neurons in the adult mammalian hippocampus,” Journal of Neuroscience, vol. 21, no. 18, pp. 7153–7160, 2001.
- L. C. Fuentealba, K. Obernier, and A. Alvarez-Buylla, “Adult neural stem cells bridge their niche,” Cell Stem Cell, vol. 10, no. 6, pp. 698–708, 2012.
- B. Steiner, F. Klempin, L. Wang, M. Kott, H. Kettenmann, and G. Kempermann, “Type-2 cells as link between glial and neuronal lineage in adult hippocampal neurogenesis,” Glia, vol. 54, no. 8, pp. 805–814, 2006.
- C. Zhao, E. M. Teng, R. G. Summers Jr., G. L. Ming, and F. H. Gage, “Distinct morphological stages of dentate granule neuron maturation in the adult mouse hippocampus,” Journal of Neuroscience, vol. 26, no. 1, pp. 3–11, 2006.
- C. Schmidt-Hieber, P. Jones, and J. Bischofberger, “Enhanced synaptic plasticity in newly generated granule cells of the adult hippocampus,” Nature, vol. 429, no. 6988, pp. 184–187, 2004.
- A. Tashiro, V. M. Sandler, N. Toni, C. Zhao, and F. H. Gage, “NMDA-receptor-mediated, cell-specific integration of new neurons in adult dentate gyrus,” Nature, vol. 442, no. 7105, pp. 929–933, 2006.
- J. Nácher, E. Varea, J. Miguel Blasco-Ibáñez et al., “N-methyl-d-aspartate receptor expression during adult neurogenesis in the rat dentate gyrus,” Neuroscience, vol. 144, no. 3, pp. 855–864, 2007.
- J. R. Epp, M. D. Spritzer, and L. A. Galea, “Hippocampus-dependent learning promotes survival of new neurons in the dentate gyrus at a specific time during cell maturation,” Neuroscience, vol. 149, no. 2, pp. 273–285, 2007.
- J. S. Snyder, N. S. Hong, R. J. McDonald, and J. M. Wojtowicz, “A role for adult neurogenesis in spatial long-term memory,” Neuroscience, vol. 130, no. 4, pp. 843–852, 2005.
- D. Dupret, J. M. Revest, M. Koehl et al., “Spatial relational memory requires hippocampal adult neurogenesis,” PLoS ONE, vol. 3, no. 4, Article ID e1959, 2008.
- W. Deng, M. D. Saxe, I. S. Gallina, and F. H. Gage, “Adult-born hippocampal dentate granule cells undergoing maturation modulate learning and memory in the brain,” Journal of Neuroscience, vol. 29, no. 43, pp. 13532–13542, 2009.
- T. Kitamura, Y. Saitoh, N. Takashima et al., “Adult neurogenesis modulates the hippocampus-dependent period of associative fear memory,” Cell, vol. 139, no. 4, pp. 814–827, 2009.
- M. D. Saxe, F. Battaglia, J. W. Wang et al., “Ablation of hippocampal neurogenesis impairs contextual fear conditioning and synaptic plasticity in the dentate gyrus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 46, pp. 17501–17506, 2006.
- C. D. Clelland, M. Choi, C. Romberg et al., “A functional role for adult hippocampal neurogenesis in spatial pattern separation,” Science, vol. 325, no. 5937, pp. 210–213, 2009.
- T. Nakashiba, J. D. Cushman, K. A. Pelkey et al., “Young dentate granule cells mediate pattern separation, whereas old granule cells facilitate pattern completion,” Cell, vol. 149, no. 1, pp. 188–201, 2012.
- J. K. Leutgeb, S. Leutgeb, M. B. Moser, and E. I. Moser, “Pattern separation in the dentate gyrus and CA3 of the hippocampus,” Science, vol. 315, no. 5814, pp. 961–966, 2007.
- A. Bakker, C. B. Kirwan, M. Miller, and C. E. Stark, “Pattern separation in the human hippocampal CA3 and dentate gyrus,” Science, vol. 319, no. 5870, pp. 1640–1642, 2008.
- M. C. McKinnon, K. Yucel, A. Nazarov, and G. M. MacQueen, “A meta-analysis examining clinical predictors of hippocampal volume in patients with major depressive disorder,” Journal of Psychiatry and Neuroscience, vol. 34, no. 1, pp. 41–54, 2009.
- J. E. Malberg, A. J. Eisch, E. J. Nestler, and R. S. Duman, “Chronic antidepressant treatment increases neurogenesis in adult rat hippocampus,” Journal of Neuroscience, vol. 20, no. 24, pp. 9104–9110, 2000.
- L. Santarelli, M. Saxe, C. Gross et al., “Requirement of hippocampal neurogenesis for the behavioral effects of antidepressants,” Science, vol. 301, no. 5634, pp. 805–809, 2003.
- T. D. Perera, A. J. Dwork, K. A. Keegan et al., “Necessity of hippocampal neurogenesis for the therapeutic action of antidepressants in adult Nonhuman primates,” PLoS ONE, vol. 6, no. 4, Article ID e17600, 2011.
- D. Petrik, D. C. Lagace, and A. J. Eisch, “The neurogenesis hypothesis of affective and anxiety disorders: are we mistaking the scaffolding for the building?” Neuropharmacology, vol. 62, no. 1, pp. 21–34, 2012.
- J. S. Snyder, A. Soumier, M. Brewer, J. Pickel, and H. A. Cameron, “Adult hippocampal neurogenesis buffers stress responses and depressive behaviour,” Nature, vol. 476, no. 7361, pp. 458–461, 2011.
- M. Maekawa, N. Takashima, M. Matsumata et al., “Arachidonic acid drives postnatal neurogenesis and elicits a beneficial effect on prepulse inhibition, a biological trait of psychiatric illnesses,” PLoS ONE, vol. 4, no. 4, Article ID e5085, 2009.
- H. van Praag, G. Kempermann, and F. H. Gage, “Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus,” Nature Neuroscience, vol. 2, no. 3, pp. 266–270, 1999.
- E. Gould, B. S. McEwen, P. Tanapat, L. A. Galea, and E. Fuchs, “Neurogenesis in the dentate gyrus of the adult tree shrew is regulated by psychosocial stress and NMDA receptor activation,” Journal of Neuroscience, vol. 17, no. 7, pp. 2492–2498, 1997.
- K. Pham, J. Nacher, P. R. Hof, and B. S. McEwen, “Repeated restraint stress suppresses neurogenesis and induces biphasic PSA-NCAM expression in the adult rat dentate gyrus,” European Journal of Neuroscience, vol. 17, no. 4, pp. 879–886, 2003.
- H. Dong, B. Goico, M. Martin, C. A. Csernansky, A. Bertchume, and J. G. Csernansky, “Modulation of hippocampal cell proliferation, memory, and amyloid plaque deposition in APPsw (Tg2576) mutant mice by isolation stress,” Neuroscience, vol. 127, no. 3, pp. 601–609, 2004.
- J. M. Encinas, T. V. Michurina, N. Peunova et al., “Division-coupled astrocytic differentiation and age-related depletion of neural stem cells in the adult hippocampus,” Cell Stem Cell, vol. 8, no. 5, pp. 566–579, 2011.
- Y. Tozuka, E. Wada, and K. Wada, “Diet-induced obesity in female mice leads to peroxidized lipid accumulations and impairment of hippocampal neurogenesis during the early life of their offspring,” The FASEB Journal, vol. 23, no. 6, pp. 1920–1934, 2009.
- A. Lindqvist, P. Mohapel, B. Bouter et al., “High-fat diet impairs hippocampal neurogenesis in male rats,” European Journal of Neurology, vol. 13, no. 12, pp. 1385–1388, 2006.
- N. Sakayori, M. Maekawa, K. Numayama-Tsuruta, T. Katura, T. Moriya, and N. Osumi, “Distinctive effects of arachidonic acid and docosahexaenoic acid on neural stem/progenitor cells,” Genes to Cells, vol. 16, no. 7, pp. 778–790, 2011.
- M. Yamato, T. Shiba, M. Yoshida et al., “Fatty acids increase the circulating levels of oxidative stress factors in mice with diet-induced obesity via redox changes of albumin,” FEBS Journal, vol. 274, no. 15, pp. 3855–3863, 2007.
- C. He, X. Qu, L. Cui, J. Wang, and J. X. Kang, “Improved spatial learning performance of fat-1 mice is associated with enhanced neurogenesis and neuritogenesis by docosahexaenoic acid,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 27, pp. 11370–11375, 2009.
- E. Kawakita, M. Hashimoto, and O. Shido, “Docosahexaenoic acid promotes neurogenesis in vitro and in vivo,” Neuroscience, vol. 139, no. 3, pp. 991–997, 2006.
- M. Katakura, M. Hashimoto, H. M. Shahdat et al., “Docosahexaenoic acid promotes neuronal differentiation by regulating basic helix-loop-helix transcription factors and cell cycle in neural stem cells,” Neuroscience, vol. 160, no. 3, pp. 651–660, 2009.
- D. Ma, M. Zhang, C. P. Larsen et al., “DHA promotes the neuronal differentiation of rat neural stem cells transfected with GPR40 gene,” Brain Research, vol. 1330, pp. 1–8, 2010.
- I. Kan, E. Melamed, D. Offen, and P. Green, “Docosahexaenoic acid and arachidonic acid are fundamental supplements for the induction of neuronal differentiation,” Journal of Lipid Research, vol. 48, no. 3, pp. 513–517, 2007.
- N. Sakayori and N. Osumi, “Polyunsaturated fatty acids and their metabolites in neural development and implications for psychiatric disorders,” Current Psychopharmacology, vol. 2, no. 1, pp. 73–83, 2013.
- T. Okui, M. Hashimoto, M. Katakura, and O. Shido, “Cis-9,trans-11-conjugated linoleic acid promotes neuronal differentiation through regulation of Hes6 mRNA and cell cycle in cultured neural stem cells,” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 85, no. 3-4, pp. 163–169, 2011.
- S. C. Dyall, G. J. Michael, and A. T. Michael-Titus, “Omega-3 fatty acids reverse age-related decreases in nuclear receptors and increase neurogenesis in old rats,” Journal of Neuroscience Research, vol. 88, no. 10, pp. 2091–2102, 2010.
- M. Soderberg, C. Edlund, K. Kristensson, and G. Dallner, “Fatty acid composition of brain phospholipids in aging and in Alzheimer's disease,” Lipids, vol. 26, no. 6, pp. 421–425, 1991.
- P. C. Bertrand, J. R. O'Kusky, and S. M. Innis, “Maternal dietary (n-3) fatty acid deficiency alters neurogenesis in the embryonic rat brain,” Journal of Nutrition, vol. 136, no. 6, pp. 1570–1575, 2006.
- P. C. Calder, “Polyunsaturated fatty acids and inflammatory processes: new twists in an old tale,” Biochimie, vol. 91, no. 6, pp. 791–795, 2009.
- K. Nakamura, T. Kaneko, Y. Yamashita, H. Hasegawa, H. Katoh, and M. Negishi, “Immunohistochemical localization of prostaglandin EP3 receptor in the rat nervous system,” Journal of Comparative Neurology, vol. 421, no. 4, pp. 543–569, 2000.
- M. Ek, C. Arias, P. Sawchenko, and A. Ericsson-Dahlstrand, “Distribution of the EP3 prostaglandin E(2) receptor subtype in the rat brain: relationship to sites of interleukin-1-induced cellular responsiveness,” Journal of Comparative Neurology, vol. 428, no. 1, pp. 5–20, 2000.
- K. Uchida, K. Kumihashi, S. Kurosawa, T. Kobayashi, K. Itoi, and T. Machida, “Stimulatory effects of prostaglandin E2 on neurogenesis in the dentate gyrus of the adult rat,” Zoological Science, vol. 19, no. 11, pp. 1211–1216, 2002.
- T. Katura, T. Moriya, and N. Nakahata, “15-Deoxy-Δ12,14-prostaglandin J2 biphasically regulates the proliferation of mouse hippocampal neural progenitor cells by modulating the redox state,” Molecular Pharmacology, vol. 77, no. 4, pp. 601–611, 2010.
- T. Ogorochi, S. Narumiya, N. Mizuno, K. Yamashita, H. Miyazaki, and O. Hayaishi, “Regional distribution of prostaglandins D2, E2, and F(2α) and related enzymes in postmortem human brain,” Journal of Neurochemistry, vol. 43, no. 1, pp. 71–82, 1984.
- G. V. Richieri, A. Anel, and A. M. Kleinfeld, “Interactions of long-chain fatty acids and albumin: determination of free fatty acid levels using the fluorescent probe ADIFAB,” Biochemistry, vol. 32, no. 29, pp. 7574–7580, 1993.
- R. Z. Liu, R. Mita, M. Beaulieu, Z. Gao, and R. Godbout, “Fatty acid binding proteins in brain development and disease,” International Journal of Developmental Biology, vol. 54, no. 8-9, pp. 1229–1239, 2010.
- Y. Owada, T. Yoshimoto, and H. Kondo, “Spatio-temporally differential expression of genes for three members of fatty acid binding proteins in developing and mature rat brains,” Journal of Chemical Neuroanatomy, vol. 12, no. 2, pp. 113–122, 1996.
- Y. Liu, L. D. Longo, and M. de León, “In situ and immunocytochemical localization of E-FABP mRNA and protein during neuronal migration and differentiation in the rat brain,” Brain Research, vol. 852, no. 1, pp. 16–27, 2000.
- Y. Owada, I. Suzuki, T. Noda, and H. Kondo, “Analysis on the phenotype of E-FABP-gene knockout mice,” Molecular and Cellular Biochemistry, vol. 239, no. 1-2, pp. 83–86, 2002.
- M. Matsumata, N. Sakayori, M. Maekawa, Y. Owada, T. Yoshikawa, and N. Osumi, “The effects of fabp7 and fabp5 on postnatal hippocampal neurogenesis in the mouse,” Stem Cells, vol. 30, no. 7, pp. 1532–1543, 2012.
- L. Feng, M. E. Hatten, and N. Heintz, “Brain lipid-binding protein (BLBP): a novel signaling system in the developing mammalian CNS,” Neuron, vol. 12, no. 4, pp. 895–908, 1994.
- A. Kurtz, A. Zimmer, F. Schnütgen, G. Brüning, F. Spener, and T. Müller, “The expression pattern of a novel gene encoding brain-fatty acid binding protein correlates with neuronal and glial cell development,” Development, vol. 120, no. 9, pp. 2637–2649, 1994.
- A. Watanabe, T. Toyota, Y. Owada et al., “Fabp7 maps to a quantitative trait locus for a schizophrenia endophenotype,” PLoS Biology, vol. 5, no. 11, article e297, 2007.
- C. D. Kane, N. R. Coe, B. Vanlandingham, P. Krieg, and D. A. Bernlohr, “Expression, purification, and ligand-binding analysis of recombinant keratinocyte lipid-binding protein (MAL-1), an intracellular lipid-binding protein found overexpressed in neoplastic skin cells,” Biochemistry, vol. 35, no. 9, pp. 2894–2900, 1996.
- T. Hanhoff, C. Lücke, and F. Spener, “Insights into binding of fatty acids by fatty acid binding proteins,” Molecular and Cellular Biochemistry, vol. 239, no. 1-2, pp. 45–54, 2002.
- L. Z. Xu, R. Sánchez, A. Sali, and N. Heintz, “Ligand specificity of brain lipid-binding protein,” The Journal of Biological Chemistry, vol. 271, no. 40, pp. 24711–24719, 1996.
- G. K. Balendiran, F. Schnütgen, G. Scapin et al., “Crystal structure and thermodynamic analysis of human brain fatty acid-binding protein,” The Journal of Biological Chemistry, vol. 275, no. 35, pp. 27045–27054, 2000.
- J.-W. Liu, F. G. Almaguel, L. Bu, D. D. de Leon, and M. de Leon, “Expression of E-FABP in PC12 cells increases neurite extension during differentiation: involvement of n-3 and n-6 fatty acids,” Journal of Neurochemistry, vol. 106, no. 5, pp. 2015–2029, 2008.
- Y. Owada, S. A. Abdelwahab, N. Kitanaka et al., “Altered emotional behavioral responses in mice lacking brain-type fatty acid-binding protein gene,” European Journal of Neuroscience, vol. 24, no. 1, pp. 175–187, 2006.
- Y. Arai, N. Funatsu, K. Numayama-Tsuruta, T. Nomura, S. Nakamura, and N. Osumi, “Role of Fabp7, a downstream gene of Pax6, in the maintenance of neuroepithelial cells during early embryonic development of the rat cortex,” Journal of Neuroscience, vol. 25, no. 42, pp. 9752–9761, 2005.
- K. Simons and W. L. Vaz, “Model systems, lipid rafts, and cell membranes,” Annual Review of Biophysics and Biomolecular Structure, vol. 33, pp. 269–295, 2004.
- J. M. Dietschy and S. D. Turley, “Cholesterol metabolism in the brain,” Current Opinion in Lipidology, vol. 12, no. 2, pp. 105–112, 2001.
- D. H. Mauch, K. Nägier, S. Schumacher et al., “CNS synaptogenesis promoted by glia-derived cholesterol,” Science, vol. 294, no. 5545, pp. 1354–1357, 2001.
- S. Suzuki, T. Numakawa, K. Shimazu et al., “BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: roles in synaptic modulation,” Journal of Cell Biology, vol. 167, no. 6, pp. 1205–1215, 2004.
- K. Röper, D. Corbeil, and W. B. Huttner, “Retention of prominin in microvilli reveals distinct cholesterol-based lipid microdomains in the apical plasma membrane,” Nature Cell Biology, vol. 2, no. 9, pp. 582–592, 2000.
- K. Saito, V. Dubreuil, Y. Arai et al., “Ablation of cholesterol biosynthesis in neural stem cells increases their VEGF expression and angiogenesis but causes neuron apoptosis,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 20, pp. 8350–8355, 2009.
- I. Y. Kim, I. K. Hwang, J. W. Choi et al., “Effects of high cholesterol diet on newly generated cells in the dentate gyrus of C57BL/6N and C3H/HeN mice,” Journal of Veterinary Medical Science, vol. 71, no. 6, pp. 753–758, 2009.
- A. Cvekl and W. L. Wang, “Retinoic acid signaling in mammalian eye development,” Experimental Eye Research, vol. 89, no. 3, pp. 280–291, 2009.
- J. Crandall, Y. Sakai, J. Zhang et al., “13-cis-retinoic acid suppresses hippocampal cell division and hippocampal-dependent learning in mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 14, pp. 5111–5116, 2004.
- S. Jacobs, D. C. Lie, K. L. DeCicco et al., “Retinoic acid is required early during adult neurogenesis in the dentate gyrus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 10, pp. 3902–3907, 2006.
- E. Bonnet, K. Touyarot, S. Alfos, V. Pallet, P. Higueret, and D. N. Abrous, “Retinoic acid restores adult hippocampal neurogenesis and reverses spatial memory deficit in vitamin A deprived rats,” PLoS ONE, vol. 3, no. 10, Article ID e3487, 2008.
- T. Cecchini, S. Ciaroni, P. Ferri et al., “α-tocopherol, an exogenous factor of adult hippocampal neurogenesis regulation,” Journal of Neuroscience Research, vol. 73, no. 4, pp. 447–455, 2003.
- S. Ciaroni, T. Cecchini, P. Ferri et al., “Neural precursor proliferation and newborn cell survival in the adult rat dentate gyrus are affected by vitamin E deficiency,” Neuroscience Research, vol. 44, no. 4, pp. 369–377, 2002.
- J. Lee, K. B. Seroogy, and M. P. Mattson, “Dietary restriction enhances neurotrophin expression and neurogenesis in the hippocampus of adult mice,” Journal of Neurochemistry, vol. 80, no. 3, pp. 539–547, 2002.
- D. Stangl and S. Thuret, “Impact of diet on adult hippocampal neurogenesis,” Genes and Nutrition, vol. 4, no. 4, pp. 271–282, 2009.
- H. Aoki, K. Kimoto, N. Hori, and M. Toyoda, “Cell proliferation in the dentate gyrus of rat hippocampus is inhibited by soft diet feeding,” Gerontology, vol. 51, no. 6, pp. 369–374, 2005.
- B. K. Puri and A. J. Richardson, “Sustained remission of positive and negative symptoms of schizophrenia following treatment with eicosapentaenoic acid,” Archives of General Psychiatry, vol. 55, no. 2, pp. 188–189, 1998.
- M. Peet, J. Brind, C. N. Ramchand, S. Shah, and G. K. Vankar, “Two double-blind placebo-controlled pilot studies of eicosapentaenoic acid in the treatment of schizophrenia,” Schizophrenia Research, vol. 49, no. 3, pp. 243–251, 2001.
- R. Emsley, C. Myburgh, P. Oosthuizen, and S. J. van Rensburg, “Randomized, placebo-controlled study of ethyl-eicosapentaenoic acid as supplemental treatment in schizophrenia,” American Journal of Psychiatry, vol. 159, no. 9, pp. 1596–1598, 2002.
- M. Peet and D. F. Horrobin, “A dose-ranging exploratory study of the effects of ethyl-eicosapentaenoate in patients with persistent schizophrenic symptoms,” Journal of Psychiatric Research, vol. 36, no. 1, pp. 7–18, 2002.
- P. Y. Lin, D. Mischoulon, M. P. Freeman, et al., “Are omega-3 fatty acids antidepressants or just mood-improving agents? The effect depends upon diagnosis, supplement preparation, and severity of depression,” Molecular Psychiatry, vol. 17, no. 12, pp. 1161–1163, 2012.
- J. G. Martins, H. Bentsen, and B. K. Puri, “Eicosapentaenoic acid appears to be the key omega-3 fatty acid component associated with efficacy in major depressive disorder: a critique of Bloch and Hannestad and updated meta-analysis,” Molecular Psychiatry, vol. 17, no. 12, pp. 1144–1149, 2012.
- M. E. Sublette, S. P. Ellis, A. L. Geant, and J. J. Mann, “Meta-analysis of the effects of eicosapentaenoic acid (EPA) in clinical trials in depression,” Journal of Clinical Psychiatry, vol. 72, no. 12, pp. 1577–1584, 2011.
- D. Nishi, Y. Koido, N. Nakaya et al., “Fish oil for attenuating posttraumatic stress symptoms among rescue workers after the great east japan earthquake: a randomized controlled trial,” Psychotherapy and Psychosomatics, vol. 81, no. 5, pp. 315–317, 2012.
- Y. Matsuoka, D. Nishi, N. Yonemoto, K. Hamazaki, K. Hashimoto, and T. Hamazaki, “Omega-3 fatty acids for secondary prevention of posttraumatic stress disorder after accidental injury an open-label pilot study,” Journal of Clinical Psychopharmacology, vol. 30, no. 2, pp. 217–219, 2010.
- Y. Matsuoka, “Clearance of fear memory from the hippocampus through neurogenesis by omega-3 fatty acids: a novel preventive strategy for posttraumatic stress disorder?” BioPsychoSocial Medicine, vol. 5, article 3, 2011.
- K. Yui, M. Koshiba, S. Nakamura, and M. Onishi, “Therapeutic effects of larger doses of arachidonic acid added to DHA on social impairment and its relation to alterations of polyunsaturated fatty acids in individuals with autism spectrum disorders,” Nihon Shinkei Seishin Yakurigaku Zasshi, vol. 31, no. 3, pp. 117–124, 2011.