Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Figure 2

Three stages of chicken iPSC-like cells morphological development in modified 2i+ media. (a) Stage 1: chicken iPSC-like cell colony during the 4th passage with a characteristic dark center. (b) Comparable colony as in (a) stained with alkaline phosphatase. (c) Stage 2: colony is lighter by the 8th passage with fibroblasts radiating from the stem cell colony. (d) Comparable colony as in (c) stained with alkaline phosphatase; the differentiated fibroblasts do not stain with alkaline phosphatase. Arrow, colony; arrowhead, fibroblast. (e) Stage 3: 10th passage, larger colony, and fewer fibroblasts underneath or on the sides. (f) Comparable colony as in (e) stained with alkaline phosphatase.