Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Figure 6

Neuronal marker staining of differentiating neurons. ((a)–(c)) Undifferentiated chicken iPSC-like cells under phase contrast (a) or with fluorescence for DAPI (b) and NeuN (c) stains. ((d)–(f)) Neuronal differentiated chicken iPSC-like cells under phase contrast (d) or with fluorescence for DAPI (e) and NeuN (f) stains at day 7. ((g)-(h)) A close-up of single cells labeled for NeuN and DAPI. In the close-up, we chose a region with fewer NeuN labeled cells in order to show the contrast between labeled and unlabeled cells. Arrow, example of strongly labeled NeuN cells; arrowhead, example of moderately labeled NeuN cells. The control iPSC-like cells had nearly no labeled cells (only 0–2 per entire well or plate).