SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate

Figure 1

Changes in the expression patterns of pluripotency markers NANOG and OCT4 are distinct from those of SOX2 in differentiating H9 cells. hES cells were treated with sodium butyrate as described in Section 2. Coexpression of pluripotency markers during differentiation on days 3 and 4 was detected by flow cytometry. Fixed and permeabilised cells were stained with anti-SSEA-3 (Alexa Fluor 488 conjugate), anti-NANOG (PE), anti-OCT4 (Alexa Fluor 647), and anti-SOX2 (PerCp Cy5.5 conjugate) antibodies as well as with DAPI. Cellular debris and doublets were excluded from analysis. Results are representative of two independent experiments.