SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate

Figure 2

Detection of pluripotency marker OCT4 and differentiation markers in differentiating H9 cells. hESC were treated with sodium butyrate as described in Section 2. Cells harvested before initiation of differentiation (day 0) and 3 or 4 days later were fixed with 1.6% PFA and permeabilised with ice-cold methanol. Coexpression of pluripotency marker OCT4 and differentiation markers on days 3 and 4 was detected by flow cytometry.