Bone Marrow-Derived Multipotent Stromal Cells Attenuate Inflammation in Obliterative Airway Disease in Mouse Tracheal Allografts
Figure 1
Isolation and trilineage differentiation of mouse bone marrow MSCs. (a)–(d) Representative flow cytometry cell sorting plots show sequential selection of cell populations as indicated by a red arrow: total adherent cells subjected to sorting (a), singlets (b), CD11b-, CD14-, CD19-, CD31-, CD34-, and CD45-cells (c), and Sca-1+ cells (d). (e)–(h) Trilineage differentiation of mouse bone marrow MSCs: Nondifferentiated MSCs in culture (e); black arrows indicate Oil Red O staining of lipid droplets within differentiated adipocytes (f); white arrows indicate staining of Collagen type II (red) produced by differentiated chondrocytes (g); and empty arrows indicate Alizarin Red staining of calcium deposit of differentiated osteocytes (h).
