Bone Marrow-Derived Multipotent Stromal Cells Attenuate Inflammation in Obliterative Airway Disease in Mouse Tracheal Allografts
Figure 3
MSCs reduce accumulation of macrophages in the allografts. To assess macrophage accumulation, grafts from each treatment group were explanted at 1 week (PBS, ; MSC, ), 2 weeks (PBS, ; MSC, ), and 4 weeks (PBS, ; MSC, ) after transplantation. (a)–(c) Tracheal sections stained with anti-Mac3 antibodies (dark brown spots) showed reduced numbers of macrophages in the MSC/IV + T-treated group at 1 week (a), 2 weeks (b), and 4 weeks (c) after transplantation when compared to the PBS-treated control group (all representative images 200x original magnification). (d) Macrophages were counted in 4 random high power fields per section. Mean counts of macrophages at 1 week, 2 weeks, and 4 weeks after transplantation in the MSC/IV + T-treated group were compared with PBS-control group. Significantly fewer macrophages were seen in the MSC/IV + T-treated group at 1 week (), 2 weeks (), and 4 weeks (). All error bars in (d) indicate SEM.
