Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation
Table 4
Kinetics of hMSC biodistribution after total body irradiation (TBI) at 3.5 Gray.
Organs (positive per mice)
Days after irradiation
3 days
7 days
15 days
30 days
60 days
90 days
120 days
Brain
0/10 (0%)
0/10 (0%)
6/8 (75%)
2/6 (33%)
4/8 (50%)
0/8 (0%)
0/6 (0%)
Heart
8/10 (80%)
6/8 (75%)
7/8 (88%)
6/8 (75%)
0/8 (0%)
4/8 (50%)
4/6 (67%)
Lung
4/10 (40%)
10/10 (100%)
8/8 (100%)
6/8 (75%)
0/8 (0%)
3/8 (38%)
6/6 (100%)
Liver
2/10 (20%)
0/10 (0%)
8/8 (100%)
4/7 (57%)
4/8 (50%)
0/8 (0%)
0/6 (0%)
Spleen
0/10 (0%)
6/8 (75%)
5/8 (63%)
4/7 (57%)
4/8 (50%)
2/6 (33%)
0/6 (0%)
Kidney
0/10 (0%)
0/10 (0%)
4/8 (50%)
3/7 (43%)
0/8 (0%)
0/8 (0%)
2/6 (33%)
Abdominal area
Muscle
2/10 (20%)
5/10 (50%)
2/6 (33%)
0/8 (0%)
0/6 (0%)
4/8 (50%)
2/6 (33%)
Stomach
4/10 (40%)
6/8 (75%)
4/7 (57%)
4/6 (67%)
0/8 (0%)
3/8 (38%)
1/6 (17%)
Duodenum
2/10 (20%)
5/10 (50%)
2/6 (33%)
0/7 (0%)
0/8 (0%)
0/8 (0%)
0/6 (0%)
Jejunum
2/10 (20%)
0/10 (0%)
2/8 (25%)
2/8 (25%)
0/8 (0%)
2/8 (25%)
0/6 (0%)
Ileum
0/10 (0%)
0/10 (0%)
0/8 (0%)
0/8 (0%)
0/8 (0%)
0/8 (0%)
0/6 (0%)
Cecum
0/10 (0%)
0/10 (0%)
3/8 (38%)
3/8 (38%)
0/8 (0%)
2/8 (25%)
2/6 (33%)
Proximal colon
0/10 (0%)
0/10 (0%)
3/8 (38%)
3/7 (43%)
0/8 (0%)
2/8 (25%)
0/6 (0%)
Distal colon
2/10 (20%)
2/8 (25%)
0/8 (0%)
1/5 (20%)
0/8 (0%)
0/8 (0%)
2/6 (33%)
Posterior leg (right)
Skin
0/10 (0%)
0/10 (0%)
3/8 (38%)
0/6 (0%)
0/8 (0%)
3/8 (38%)
0/6 (0%)
Muscle
0/10 (0%)
5/10 (50%)
7/8 (88%)
2/8 (25%)
4/8 (50%)
0/8 (0%)
0/6 (0%)
Bone
0/10 (0%)
2/8 (25%)
1/8 (12.5%)
0/8 (0%)
4/8 (50%)
2/8 (25%)
0/6 (0%)
Bone marrow
0/10 (0%)
1/8 (12.5%)
5/8 (63%)
3/8 (38%)
0/8 (0%)
0/8 (0%)
1/6 (17%)
Blood
0/10 (0%)
0/10 (0%)
1/8 (12.5%)
4/7 (57%)
0/8 (0%)
0/8 (0%)
0/6 (0%)
Number of positive organs
26/190
48/178
71/151
47/137
40/150
27/150
20/114
Frequency
14%
28%
47%
34%
27%
18%
18%
The time at which the implantation of hMSCs is optimum varies from one organ to another. hMSCs were detectable at least up to 120 days after injection of human cells in a number of organs. Abdominal irradiation as compared to TBI modifies hMSC engraftment in the exposed area. Abdominal irradiation increased hMSC engraftment from 3 to 60 days in the stomach, duodenum, jejunum, and ileum and decreased engraftment in cecum and colon.
