Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells

Figure 1

T-MSCs inhibit differentiation and maturation of DCs under coculture conditions. Bone-marrow- (BM-) derived monocytes (BMCs) isolated from 8-week-old BALB/c mice were incubated with of granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng/mL) for 12 days to induce differentiation into dendritic cells (DCs). (a) Lipopolysaccharide (LPS) (1 μg/mL) was added for the last 2 days to induce DC maturation. (b) GM-CSF treatment induced expansion of CD11b⁺ cells (). (c) Tonsil-derived MSCs (T-MSCs) added at day 0, but not at day 10, inhibited upregulated expression of CD80 () and CD86 () on mature DCs by direct contact. Upregulated major histocompatibility complex (MHC) class II expression on immature () and mature DCs () was reduced by T-MSCs, but CD14 expression was not affected.