Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells

Figure 3

T-MSCs inhibit DC-mediated T cell proliferation and differentiation of the CD4⁺ T cell subset. (a) T cells were isolated from mouse spleens (BALB/c, 8-week-old, female) using MACS. (b) T cells were cocultured with mature DCs in the presence or absence of T-MSCs, and differentiation into CD4⁺ T cell subsets was induced using anti-CD3 and anti-CD28 antibodies. T cell numbers were increased upon coculturing with mature DCs. In the presence of T-MSCs, however, T cell proliferation was inhibited by 80%. (c) Differentiation into Th1, Th2, and Th17 cells also was decreased upon addition of T-MSCs.