Research Article
Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate
Table 1
Primer sequence used for semiquantitative RT-PCR analysis.
| | Gene | Forward primer | Reverse primer | Ta | Size | |
°C | (bp) |
| | 18S | 5′-CGGCTACCACATCCAAGGAA-3′ | 5′-GCTGGAATTACCGCGGCT-3′ | 56 | 186 | | CD31 | 5′-CAGGGTGACACTGGACAAGA-3′ | 5′-GGAGCAGGACAGGTTCAGTC-3′ | 59 | 650 | | CD34 | 5′-AATGAGGCCACAACAAACATCACA-3′ | 5′CTGTCCTTCTTAACCTCCGCACAGC-3′ | 57 | 400 | | VEGF-A | 5′-CGAAGTGGTGAAGTTCATGGATG-3′ | 5′TTCTGTATCAGTCTTTCCTGGTGAG-3′ | 60 | 476 | | IGF-1 | 5′-GACATGCCCAAGACCCAGAAGGA-3′ | 5′-CGGTGGCATGTCACTCTTCACTC-3′ | 63 | 118 | | HGF | 5′-ATGCATCCAAGGTCAAGGAG-3′ | 5′-TTCCATGTTCTTGTCCCACA-3′ | 61 | 349 | | v-WF | 5′-TAAGTCTGAAGTAGAGGTGG-3′ | 5′-AGAGCAGCAGGAGCACTGGT-3′ | 59 | 100 | | VE-Cad | 5′-GTGGAAGCGCGAGATGCCCA-3′ | 5′-AGCGTCCTGGTAGTCGCCCC-3′ | 59 | 237 |
|
|
Ta: annealing temperature; bp: base pair.
|
