Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis

Figure 1

Murine MSCs characterization. (a) Phenotypic characterization of MSCs. Cells were stained for classical murine MSCs markers (CD45, CD34, CD44, CD90, CD29, and Sca-1) and evaluated by flow cytometry using a Beckman Coulter Epics XL and FCS Express V4 Plus Research Edition software. Representative histograms for each antigen are shown in black and overlapping isotype controls in grey. (b) Immunosuppressive capacity of MSCs. Splenocytes were CFSE-stained and stimulated with Concanavalin A (3 μg/mL) (ConA) in the presence or absence of MSCs for 5 days. Nonstimulated cells were used as negative controls. T cell proliferation was evaluated by flow cytometry, gating on CD4+ cells. Results are shown as the mean ± SEM. values were calculated using the unpaired Mann-Whitney test (), .

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