Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis

Figure 6

Cytokines levels in plasma samples of EAE mice at the different times of EAE progression. Blood samples were taken at different times of EAE progression, before EAE induction (day 0), at the onset (day +10), before the peak (day +16), after the peak (day +22), at the time of EAE stabilization (day +30), after EAE stabilization (day +36), and at the end of experiment (day +50). Plasma samples were isolated by centrifugation and stored at −80°C until being used. Cytokines levels were detected using Milliplex mouse Th17 magnetic bead panel on Luminex 200 instrument (Merck, Millipore) and the kinetics were plotted of (a) Th1, (b) Th17 cytokines profiles and (c) IL-4 and IL-10 of EAE mice (, each experiment). The samples of each experiment were pooled and analyzed independently in triplicate.

(a)

(b)

(c)