Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies

Figure 5

wRMSD analysis of gene expression profiles of reprogramming-related genes between 4F2A if-iPSCs and NGFP if-iPSCs. (a) GEPs of reprogramming-related genes in individual colonies. Spline-type plots are shown where individual lines in different colors denote the expression profiles of reprogramming-related genes in individual colonies. (b) Mean GEPs of individual colony groups. Error bars, standard error. (c) Relative wRMSD. It is obtained by assessing the deviations of the gene expression in each NGFP if-iPSC and 4F2A if-iPSC colony from the mean GEP of the mESC group. A reference line in red on each panel denotes the mean wRMSD in the mESC colony group. (d) Statistical difference between the groups. The values are indicated above (-test). The wRMSDs of individual colonies are shown as crosses on the left of each box plot. The wRMSD range is set to 0.0–2.0 for a group-to-group comparison, and those wRMSDs beyond 2.0 are denoted above. The mean values are indicated in the boxes (circles). Box whiskers indicate the range from 5% to 95%.

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