A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence

Figure 5

Genes identified by expression arrays and comparative analyses are downregulated during TSC differentiation when validated by qPCR. mRNA expression levels for genes selected by dataset comparisons were quantified at 24 h intervals by qRT-PCR of undifferentiated FACS-enriched TSCs at day 0 (d0) and after forced differentiation for three days (d1–d3). Expression levels are depicted as means of biological triplicates relative to d0, which was set to 1. All genes were significantly ( value ≤ 0.05) downregulated at different time points unless indicated otherwise (n.s., not significant). Error bars indicate the standard error of the mean. values were calculated according to two-sided Student’s -test. All marker genes show grossly reduced expression during the course of 3 days of differentiation.