Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro

Figure 3

Immunocytochemical quantification of skeletal and cardiac muscle markers in MDSCs cultured under dynamic support. Cell clusters from the initial cell population (ISH0) and after applying different cell culture conditions for 12 days (I12, S12, and H12) were dissociated, plated, and stained for desmin, cTnT, Nkx2.5, Pax7, Myf3, Myf4, ACTN2, and Gata4. Ratios were calculated as positive cells (stained for marker) versus total cells (stained for DAPI). .