Improvement of In Vitro Osteogenic Potential through Differentiation of Induced Pluripotent Stem Cells from Human Exfoliated Dental Tissue towards Mesenchymal-Like Stem Cells

Figure 1

(a) Morphology of undifferentiated hiPSC colonies cultured on matrigel and MSC-like cells from iPS-SHED and iPS-FIB after 12 days of in vitro mesenchymal induction. Scale bar = 100 um. (b) Real-time quantitative PCR analysis of pluripotency markers in undifferentiated hiPSCs ((b′) SHED and (b′′) fibroblasts) and in MSC-like cells from iPS-SHED and from iPS-FIB. ACTB, TBP, and HMBS were used as endogenous controls. Values represent means +/− SD, (), (), and ().

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