Stem Cells International

Research Article

Improvement of In Vitro Osteogenic Potential through Differentiation of Induced Pluripotent Stem Cells from Human Exfoliated Dental Tissue towards Mesenchymal-Like Stem Cells

Table 1

Primers used for real-time quantitative PCR experiments.
TargetNMForward primerReverse primer
OCT3 (pluripotency)NM_001173531.1gtggtcagccaactcgtcaccaaaaaccctggcacaaact
OCT4 (pluripotency)NM_002701.3cctcacttcactgcactgtacaggttttctttccctagct
NANOG (pluripotency)NM_024865tggacactggctgaatccttccgttgattaggctccaaccat
RUNX2 NM_001024630.3agtggacgaggcaagagtttcgttcccgaggtccatctactg
ALP NM_000478.4gatacaagcactcccacttcatctgctgttcagctcgtactgcatgtc
BGLAP NM_199173ggcgctacctgtatcaatgggtggtcagccaactcgtca
COL1A1 NM_000088.3gggccaagacgaagacatcaacacccttgccgttgtcg
DLX5 NM_005221accagccagaagaagtgacccttctctgtaatgcggcca
CD105 (ENG)NM_001144950tgcacttggcctacaattccaagctgcccactcaaggatct
ACTB (endogenous control)NM_001101tgaagtgtgacgtggacatcggaggagcaatgatcttgat
TBP (endogenous control)NM_001172085gtgacccagcatcactgtttcgcaaaccagaaacccttgcg
HMBS (endogenous control)NM_001024382agcttgctcgcatacagacgagctccttggtaaacaggctt