Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro

Figure 5

Neurite outgrowth by PC12 cells was observed in different conditions. PC12 cells were labelled by CM-Dil (red) and SC-like cells or REMSCs were infected with GFP virus (green). (a) PC12 cells were cultured alone in DF12 medium. (b) PC12 cells were cultured in SCDM alone. (c) PC12 cells were cocultured with REMSCs in DF12 medium. (d) PC12 cells were cocultured with SC-like cells in SCDM. PC12 cells with few neurites in (a) and (b) were round red. PC12 cells with neurites were observed in (c) and (d), and the cells in (d) showed morphological phenotype of mature neuron. The percentage of cells with neurites (e) and the length of neurites (f) were shown in the two bar graphs, respectively. Data were presented as mean ± SEM from three independent experiments. represent significant differences compared between group C or group D and group A; ^## represent significant differences compared between groups C and D. Bar: 50 μm for all figures.

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