Electrospun Gelatin/-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca²⁺-Sensing Receptor Signaling

Figure 5

In vitro bioactivity of rBMSCs on nanofibrous scaffolds. ((a) and (b)) SEM images of rBMSCs seeded on (a) gelatin and (b) gelatin/β-TCP composite scaffolds after 24 h of culture. Insets show representative images of the cytoskeleton. (c) Proliferation of rBMSCs grown on various scaffolds as assessed by a CCK-8 assay. (d) The measured cell spreading areas. (e) Alkaline phosphatase (ALP) activity of rBMSCs cultured on various scaffolds at 4, 7, and 14 days.

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