Mesenchymal Stem Cells for Cardiac Regenerative Therapy: Optimization of Cell Differentiation Strategy

Figure 1

The diagram for the induction and identification of cardiomyocyte-like cells. MSCs cultured in medium supplemented with 5-Aza, DMSO, and BMP-2 will be induced to cardiomyocyte-like cells 24 h later. MSCs incubated in CLM/myocardial cell broth will differentiate to cardiomyocyte-like cells after 2 w. MSCs cocultured with cardiomyocyte will differentiated to cardiomyocyte-like cells 7 d later. The identification methods consist of morphology detection and molecular marker analysis.